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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methionyl-tRNA formyltransferase (fmt) CSB-YP015150RND
CSB-EP015150RND
CSB-BP015150RND
CSB-MP015150RND
CSB-EP015150RND-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Tu (tuf1) CSB-YP025342FNW
CSB-EP025342FNW
CSB-BP025342FNW
CSB-MP025342FNW
CSB-EP025342FNW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-galactosidase (lacZ), partial CSB-YP009476FLV
CSB-EP009476FLV
CSB-BP009476FLV
CSB-MP009476FLV
CSB-EP009476FLV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema hyodysenteriae Acyl carrier protein (acpP) CSB-YP015636TPQ
CSB-EP015636TPQ
CSB-BP015636TPQ
CSB-MP015636TPQ
CSB-EP015636TPQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 6-phosphofructokinase (pfkA) CSB-YP017822BRJ
CSB-EP017822BRJ
CSB-BP017822BRJ
CSB-MP017822BRJ
CSB-EP017822BRJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mus dunni Proteasome subunit beta type-9 (Psmb9) CSB-YP018887MUV
CSB-EP018887MUV
CSB-BP018887MUV
CSB-MP018887MUV
CSB-EP018887MUV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Cyclin-dependent kinase 17 (Cdk17) CSB-YP017649RA
CSB-EP017649RA
CSB-BP017649RA
CSB-MP017649RA
CSB-EP017649RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Calcipressin-1 (RCAN1) CSB-YP019500DXU
CSB-EP019500DXU
CSB-BP019500DXU
CSB-MP019500DXU
CSB-EP019500DXU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes Hepatocyte nuclear factor 3-beta (foxa2) CSB-YP008795OFH
CSB-EP008795OFH
CSB-BP008795OFH
CSB-MP008795OFH
CSB-EP008795OFH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Fibulin-1 (fbln1), partial CSB-YP008452DIL
CSB-EP008452DIL
CSB-BP008452DIL
CSB-MP008452DIL
CSB-EP008452DIL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meleagris gallopavo Growth/differentiation factor 8 (MSTN) CSB-YP015057MQV
CSB-EP015057MQV
CSB-BP015057MQV
CSB-MP015057MQV
CSB-EP015057MQV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Guanine nucleotide-binding protein subunit beta-2-like 1 (gnb2l1) CSB-YP009605DIL
CSB-EP009605DIL
CSB-BP009605DIL
CSB-MP009605DIL
CSB-EP009605DIL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Flotillin-2 (flot2) CSB-YP008728DQG
CSB-EP008728DQG
CSB-BP008728DQG
CSB-MP008728DQG
CSB-EP008728DQG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio E3 ubiquitin-protein ligase Mdm2 (mdm2) CSB-YP013626DIL
CSB-EP013626DIL
CSB-BP013626DIL
CSB-MP013626DIL
CSB-EP013626DIL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Beta-1 adrenergic receptor (adrb1), partial CSB-YP001391XBE1
CSB-EP001391XBE1
CSB-BP001391XBE1
CSB-MP001391XBE1
CSB-EP001391XBE1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Corticotropin-releasing factor receptor 2 (crhr2), partial CSB-YP005966XBE
CSB-EP005966XBE
CSB-BP005966XBE
CSB-MP005966XBE
CSB-EP005966XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Serine/threonine-protein kinase rio1 (rio1) CSB-YP019732SXV
CSB-EP019732SXV
CSB-BP019732SXV
CSB-MP019732SXV
CSB-EP019732SXV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Pterin-4-alpha-carbinolamine dehydratase (omt2) CSB-YP017514SXV
CSB-EP017514SXV
CSB-BP017514SXV
CSB-MP017514SXV
CSB-EP017514SXV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Tetratricopeptide repeat protein 1 (tpr1), partial CSB-YP025203SXV
CSB-EP025203SXV
CSB-BP025203SXV
CSB-MP025203SXV
CSB-EP025203SXV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Anaphase-promoting complex subunit 4 (cut20), partial CSB-YP001699SXV
CSB-EP001699SXV
CSB-BP001699SXV
CSB-MP001699SXV
CSB-EP001699SXV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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