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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhizobium meliloti Methylmalonyl-CoA mutase (bhbA), partial CSB-YP015243RKU
CSB-EP015243RKU
CSB-BP015243RKU
CSB-MP015243RKU
CSB-EP015243RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UDP-glucose 6-dehydrogenase (udg) CSB-YP025567EZX
CSB-EP025567EZX
CSB-BP025567EZX
CSB-MP025567EZX
CSB-EP025567EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Proto-oncogene c-Fos (FOS) CSB-YP008790MRG
CSB-EP008790MRG
CSB-BP008790MRG
CSB-MP008790MRG
CSB-EP008790MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Melatonin-related receptor (Gpr50), partial CSB-YP009817MO1
CSB-EP009817MO1
CSB-BP009817MO1
CSB-MP009817MO1
CSB-EP009817MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Vasopressin V2 receptor (Avpr2), partial CSB-YP002470MO1
CSB-EP002470MO1
CSB-BP002470MO1
CSB-MP002470MO1
CSB-EP002470MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Baculoviral IAP repeat-containing protein 6 (Birc6), partial CSB-YP002707MO
CSB-EP002707MO
CSB-BP002707MO
CSB-MP002707MO
CSB-EP002707MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Frizzled-8 (fzd8), partial CSB-YP009111XBE1
CSB-EP009111XBE1
CSB-BP009111XBE1
CSB-MP009111XBE1
CSB-EP009111XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Notothenia coriiceps neglecta L-lactate dehydrogenase A chain (ldha) CSB-YP012832NHX
CSB-EP012832NHX
CSB-BP012832NHX
CSB-MP012832NHX
CSB-EP012832NHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paranotothenia magellanica L-lactate dehydrogenase A chain (ldha) CSB-YP012832ERY
CSB-EP012832ERY
CSB-BP012832ERY
CSB-MP012832ERY
CSB-EP012832ERY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Astyanax fasciatus Alpha-crystallin A chain (cryaa) CSB-YP006007DPH
CSB-EP006007DPH
CSB-BP006007DPH
CSB-MP006007DPH
CSB-EP006007DPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 2 (fusA), partial CSB-YP007434MSO
CSB-EP007434MSO
CSB-BP007434MSO
CSB-MP007434MSO
CSB-EP007434MSO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum aerophilum Methylated-DNA--protein-cysteine methyltransferase (ogt) CSB-YP013788FHU
CSB-EP013788FHU
CSB-BP013788FHU
CSB-MP013788FHU
CSB-EP013788FHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus kodakaraensis Aspartate aminotransferase (aspC) CSB-YP009681FHY
CSB-EP009681FHY
CSB-BP009681FHY
CSB-MP009681FHY
CSB-EP009681FHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans DNA polymerase epsilon catalytic subunit A (pol2), partial CSB-YP018309EKF
CSB-EP018309EKF
CSB-BP018309EKF
CSB-MP018309EKF
CSB-EP018309EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans MutS protein homolog 4 (MSH4), partial CSB-YP015030CZF
CSB-EP015030CZF
CSB-BP015030CZF
CSB-MP015030CZF
CSB-EP015030CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colletotrichum gloeosporioides Superoxide dismutase [Cu-Zn] (SOD1) CSB-YP022397DVE
CSB-EP022397DVE
CSB-BP022397DVE
CSB-MP022397DVE
CSB-EP022397DVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Putative ribosomal RNA methyltransferase nop2 (SPBP8B7.20c), partial CSB-YP015938SXV
CSB-EP015938SXV
CSB-BP015938SXV
CSB-MP015938SXV
CSB-EP015938SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe RNA exonuclease 4 (rex4) CSB-YP019587SXV
CSB-EP019587SXV
CSB-BP019587SXV
CSB-MP019587SXV
CSB-EP019587SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Replication factor C subunit 4 (rfc4) CSB-YP019591SXV
CSB-EP019591SXV
CSB-BP019591SXV
CSB-MP019591SXV
CSB-EP019591SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Nucleolar protein 56 (nop56) CSB-YP015939SXV
CSB-EP015939SXV
CSB-BP015939SXV
CSB-MP015939SXV
CSB-EP015939SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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