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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus gordonii GTPase Era (era) CSB-YP007759FMN
CSB-EP007759FMN
CSB-BP007759FMN
CSB-MP007759FMN
CSB-EP007759FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Nucleoside diphosphate kinase (ndk) CSB-YP015889MSR
CSB-EP015889MSR
CSB-BP015889MSR
CSB-MP015889MSR
CSB-EP015889MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Methylated-DNA--protein-cysteine methyltransferase (ogt) CSB-YP013788MSR
CSB-EP013788MSR
CSB-BP013788MSR
CSB-MP013788MSR
CSB-EP013788MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Amidophosphoribosyltransferase (purF) CSB-YP018427MSR
CSB-EP018427MSR
CSB-BP018427MSR
CSB-MP018427MSR
CSB-EP018427MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus tRNA pseudouridine synthase A (truA) CSB-YP019086MSR
CSB-EP019086MSR
CSB-BP019086MSR
CSB-MP019086MSR
CSB-EP019086MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Argininosuccinate synthase (argG) CSB-YP002234MSR
CSB-EP002234MSR
CSB-BP002234MSR
CSB-MP002234MSR
CSB-EP002234MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus TATA-box-binding protein (tbp) CSB-YP023239MSR
CSB-EP023239MSR
CSB-BP023239MSR
CSB-MP023239MSR
CSB-EP023239MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Protein pelota homolog (pelA) CSB-YP017778MSR
CSB-EP017778MSR
CSB-BP017778MSR
CSB-MP017778MSR
CSB-EP017778MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Delta-aminolevulinic acid dehydratase (hemB) CSB-YP001558DOC
CSB-EP001558DOC
CSB-BP001558DOC
CSB-MP001558DOC
CSB-EP001558DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus DNA topoisomerase 1 (topA), partial CSB-YP024056DOC
CSB-EP024056DOC
CSB-BP024056DOC
CSB-MP024056DOC
CSB-EP024056DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Ribose-phosphate pyrophosphokinase 2 (prs2) CSB-YP018779DOC
CSB-EP018779DOC
CSB-BP018779DOC
CSB-MP018779DOC
CSB-EP018779DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Triosephosphate isomerase (tpiA) CSB-YP024102DOC
CSB-EP024102DOC
CSB-BP024102DOC
CSB-MP024102DOC
CSB-EP024102DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Protein pelota homolog (pelA) CSB-YP017778DOC
CSB-EP017778DOC
CSB-BP017778DOC
CSB-MP017778DOC
CSB-EP017778DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus D-3-phosphoglycerate dehydrogenase (serA), partial CSB-YP017920DOC
CSB-EP017920DOC
CSB-BP017920DOC
CSB-MP017920DOC
CSB-EP017920DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Flap endonuclease 1 (fen) CSB-YP008585DOC
CSB-EP008585DOC
CSB-BP008585DOC
CSB-MP008585DOC
CSB-EP008585DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida tRNA dimethylallyltransferase (miaA) CSB-YP024529FFZ
CSB-EP024529FFZ
CSB-BP024529FFZ
CSB-MP024529FFZ
CSB-EP024529FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti NADP-dependent malic enzyme (tme), partial CSB-YP013634RKU
CSB-EP013634RKU
CSB-BP013634RKU
CSB-MP013634RKU
CSB-EP013634RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allochromatium vinosum Cytochrome b (petB), partial CSB-YP015075AZP
CSB-EP015075AZP
CSB-BP015075AZP
CSB-MP015075AZP
CSB-EP015075AZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triosephosphate isomerase (tpiA) CSB-YP024102LMM
CSB-EP024102LMM
CSB-BP024102LMM
CSB-MP024102LMM
CSB-EP024102LMM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Thioredoxin reductase (trxB) CSB-YP025388LPY
CSB-EP025388LPY
CSB-BP025388LPY
CSB-MP025388LPY
CSB-EP025388LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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