Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter sp. ATP synthase subunit delta (atpH) CSB-YP002355GFR
CSB-EP002355GFR
CSB-BP002355GFR
CSB-MP002355GFR
CSB-EP002355GFR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurus punctatus Protein NLRC5 (nlrc5), partial CSB-YP015863ILX
CSB-EP015863ILX
CSB-BP015863ILX
CSB-MP015863ILX
CSB-EP015863ILX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-galactosidase, partial CSB-YP009476DOJ
CSB-EP009476DOJ
CSB-BP009476DOJ
CSB-MP009476DOJ
CSB-EP009476DOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Peroxisome assembly protein 12 (PEX12), partial CSB-YP017798EKF1
CSB-EP017798EKF1
CSB-BP017798EKF1
CSB-MP017798EKF1
CSB-EP017798EKF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Methionine aminopeptidase 2 (MAP2) CSB-YP013716SVN
CSB-EP013716SVN
CSB-BP013716SVN
CSB-MP013716SVN
CSB-EP013716SVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verticillium albo-atrum Enolase-phosphatase E1 (UTR4) CSB-YP007674VES
CSB-EP007674VES
CSB-BP007674VES
CSB-MP007674VES
CSB-EP007674VES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trypanosoma brucei gambiense Flap endonuclease 1 (FEN1) CSB-YP008585TQW
CSB-EP008585TQW
CSB-BP008585TQW
CSB-MP008585TQW
CSB-EP008585TQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trypanosoma brucei gambiense Methylthioribose-1-phosphate isomerase (TbgDal_XI12320) CSB-YP014799TQW
CSB-EP014799TQW
CSB-BP014799TQW
CSB-MP014799TQW
CSB-EP014799TQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Cytosolic carboxypeptidase-like protein 5 (AGBL5), partial CSB-YP001439AYX
CSB-EP001439AYX
CSB-BP001439AYX
CSB-MP001439AYX
CSB-EP001439AYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca E3 ubiquitin-protein ligase RNF146 (RNF146) CSB-YP019843AYX
CSB-EP019843AYX
CSB-BP019843AYX
CSB-MP019843AYX
CSB-EP019843AYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Wee1-like protein kinase 2 (WEE2), partial CSB-YP026085AYX
CSB-EP026085AYX
CSB-BP026085AYX
CSB-MP026085AYX
CSB-EP026085AYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Serine/threonine-protein kinase DCLK2 (DCLK2), partial CSB-YP006549AYX
CSB-EP006549AYX
CSB-BP006549AYX
CSB-MP006549AYX
CSB-EP006549AYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naegleria gruberi Enolase-phosphatase E1 (NAEGRDRAFT_60166) CSB-YP007674NFF
CSB-EP007674NFF
CSB-BP007674NFF
CSB-MP007674NFF
CSB-EP007674NFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polysphondylium pallidum U1 small nuclear ribonucleoprotein C (snrpC) CSB-YP022331EXO
CSB-EP022331EXO
CSB-BP022331EXO
CSB-MP022331EXO
CSB-EP022331EXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polysphondylium pallidum Flap endonuclease 1 (repG) CSB-YP008585EXO
CSB-EP008585EXO
CSB-BP008585EXO
CSB-MP008585EXO
CSB-EP008585EXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenobacter thermophilus Serine hydroxymethyltransferase (glyA) CSB-YP021274HXS
CSB-EP021274HXS
CSB-BP021274HXS
CSB-MP021274HXS
CSB-EP021274HXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter ruminantium FAD synthase (ribL) CSB-YP008710MRR
CSB-EP008710MRR
CSB-BP008710MRR
CSB-MP008710MRR
CSB-EP008710MRR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobrevibacter ruminantium Elongation factor 2 (fusA), partial CSB-YP007434MRR
CSB-EP007434MRR
CSB-BP007434MRR
CSB-MP007434MRR
CSB-EP007434MRR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Ribonuclease 3 (rnc), partial CSB-YP019809MWH
CSB-EP019809MWH
CSB-BP019809MWH
CSB-MP019809MWH
CSB-EP019809MWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Ribonuclease 3 (rnc), partial CSB-YP019809MWG
CSB-EP019809MWG
CSB-BP019809MWG
CSB-MP019809MWG
CSB-EP019809MWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>