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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Signal recognition particle 14 kDa protein (SRP14) CSB-YP022673DOA
CSB-EP022673DOA
CSB-BP022673DOA
CSB-MP022673DOA
CSB-EP022673DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium acetobutylicum Methionyl-tRNA formyltransferase (fmt) CSB-YP015150DUB
CSB-EP015150DUB
CSB-BP015150DUB
CSB-MP015150DUB
CSB-EP015150DUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoenolpyruvate carboxykinase [GTP] (pckG), partial CSB-YP017614MVN
CSB-EP017614MVN
CSB-BP017614MVN
CSB-MP017614MVN
CSB-EP017614MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146HUV
CSB-EP019146HUV
CSB-BP019146HUV
CSB-MP019146HUV
CSB-EP019146HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Sodium channel protein type 9 subunit alpha (Scn9a), partial CSB-YP020846RA
CSB-EP020846RA
CSB-BP020846RA
CSB-MP020846RA
CSB-EP020846RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Gamma-sarcoglycan (SGCG), partial CSB-YP021186MRG1
CSB-EP021186MRG1
CSB-BP021186MRG1
CSB-MP021186MRG1
CSB-EP021186MRG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Growth hormone secretagogue receptor type 1 (Ghsr), partial CSB-YP009416RA1
CSB-EP009416RA1
CSB-BP009416RA1
CSB-MP009416RA1
CSB-EP009416RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Progonadoliberin-1 (GNRH1) CSB-YP009635MRG
CSB-EP009635MRG
CSB-BP009635MRG
CSB-MP009635MRG
CSB-EP009635MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Presenilin-1 (psen1), partial CSB-YP018846XBE
CSB-EP018846XBE
CSB-BP018846XBE
CSB-MP018846XBE
CSB-EP018846XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Columba livia Alpha-crystallin B chain (CRYAB) CSB-YP006008DVK
CSB-EP006008DVK
CSB-BP006008DVK
CSB-MP006008DVK
CSB-EP006008DVK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Sodium/potassium-transporting ATPase subunit gamma (fxyd2), partial CSB-YP009090XBE1
CSB-EP009090XBE1
CSB-BP009090XBE1
CSB-MP009090XBE1
CSB-EP009090XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ran GTPase-activating protein 1 (rangap1), partial CSB-YP019318XBE
CSB-EP019318XBE
CSB-BP019318XBE
CSB-MP019318XBE
CSB-EP019318XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Ephrin type-A receptor 3 (epha3), partial CSB-YP007723DIL
CSB-EP007723DIL
CSB-BP007723DIL
CSB-MP007723DIL
CSB-EP007723DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Decapterus maruadsi Hemoglobin subunit beta (hbb) CSB-YP010150DIT
CSB-EP010150DIT
CSB-BP010150DIT
CSB-MP010150DIT
CSB-EP010150DIT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Devario pathirana Indian hedgehog protein (ihh) CSB-YP011569DJZ
CSB-EP011569DJZ
CSB-BP011569DJZ
CSB-MP011569DJZ
CSB-EP011569DJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sonic hedgehog protein (shh) CSB-YP021266DIJ
CSB-EP021266DIJ
CSB-BP021266DIJ
CSB-MP021266DIJ
CSB-EP021266DIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphyraena lucasana L-lactate dehydrogenase A chain (ldha) CSB-YP012832FKG
CSB-EP012832FKG
CSB-BP012832FKG
CSB-MP012832FKG
CSB-EP012832FKG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Importin subunit beta-1 (kap95), partial CSB-YP012490SXV
CSB-EP012490SXV
CSB-BP012490SXV
CSB-MP012490SXV
CSB-EP012490SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe U3 small nucleolar ribonucleoprotein protein mpp10 (mpp10), partial CSB-YP014750SXV
CSB-EP014750SXV
CSB-BP014750SXV
CSB-MP014750SXV
CSB-EP014750SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Diphosphomevalonate decarboxylase (mvd1) CSB-YP015246SXV
CSB-EP015246SXV
CSB-BP015246SXV
CSB-MP015246SXV
CSB-EP015246SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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