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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Tachyglossus aculeatus aculeatus Amelogenin (AMEL) CSB-YP001664TKU
CSB-EP001664TKU
CSB-BP001664TKU
CSB-MP001664TKU
CSB-EP001664TKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichosurus vulpecula Interleukin-10 (IL10) CSB-YP011580TQG
CSB-EP011580TQG
CSB-BP011580TQG
CSB-MP011580TQG
CSB-EP011580TQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachypithecus francoisi C-C chemokine receptor type 5 (CCR5), partial CSB-YP004844TOZ1
CSB-EP004844TOZ1
CSB-BP004844TOZ1
CSB-MP004844TOZ1
CSB-EP004844TOZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pygathrix nemaeus C-C chemokine receptor type 5 (CCR5), partial CSB-YP004844FHO1
CSB-EP004844FHO1
CSB-BP004844FHO1
CSB-MP004844FHO1
CSB-EP004844FHO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vulpes vulpes Kinectin (KTN1), partial CSB-YP012700VFW
CSB-EP012700VFW
CSB-BP012700VFW
CSB-MP012700VFW
CSB-EP012700VFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colobus polykomos Cytochrome c oxidase subunit 1 (MT-CO1), partial CSB-YP015072DVI
CSB-EP015072DVI
CSB-BP015072DVI
CSB-MP015072DVI
CSB-EP015072DVI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome b (MT-CYB), partial CSB-YP015075EWN
CSB-EP015075EWN
CSB-BP015075EWN
CSB-MP015075EWN
CSB-EP015075EWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alcelaphus lichtensteinii Cytochrome b (MT-CYB), partial CSB-YP015075AZI
CSB-EP015075AZI
CSB-BP015075AZI
CSB-MP015075AZI
CSB-EP015075AZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kobus ellipsiprymnus Cytochrome b (MT-CYB), partial CSB-YP015075KBL
CSB-EP015075KBL
CSB-BP015075KBL
CSB-MP015075KBL
CSB-EP015075KBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artibeus jamaicensis NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP015080DOQ1
CSB-EP015080DOQ1
CSB-BP015080DOQ1
CSB-MP015080DOQ1
CSB-EP015080DOQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eulemur mongoz Cytochrome b (MT-CYB), partial CSB-YP015075EPH
CSB-EP015075EPH
CSB-BP015075EPH
CSB-MP015075EPH
CSB-EP015075EPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhipicephalus sanguineus NADH-ubiquinone oxidoreductase chain 2 (ND2), partial CSB-YP015077RKI
CSB-EP015077RKI
CSB-BP015077RKI
CSB-MP015077RKI
CSB-EP015077RKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhipicephalus sanguineus ATP synthase subunit a (ATP6), partial CSB-YP015070RKI1
CSB-EP015070RKI1
CSB-BP015070RKI1
CSB-MP015070RKI1
CSB-EP015070RKI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhipicephalus sanguineus NADH-ubiquinone oxidoreductase chain 4 (ND4), partial CSB-YP015079RKI
CSB-EP015079RKI
CSB-BP015079RKI
CSB-MP015079RKI
CSB-EP015079RKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhipicephalus sanguineus Cytochrome b (MT-CYB), partial CSB-YP015075RKI
CSB-EP015075RKI
CSB-BP015075RKI
CSB-MP015075RKI
CSB-EP015075RKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe tRNA-specific adenosine deaminase 1 (SPBC16A3.06) CSB-YP001327SXV
CSB-EP001327SXV
CSB-BP001327SXV
CSB-MP001327SXV
CSB-EP001327SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mannose-6-phosphate isomerase (pmi40) CSB-YP014754SXV
CSB-EP014754SXV
CSB-BP014754SXV
CSB-MP014754SXV
CSB-EP014754SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Vacuolar protein-sorting-associated protein 36 (vps36) CSB-YP025905SXV
CSB-EP025905SXV
CSB-BP025905SXV
CSB-MP025905SXV
CSB-EP025905SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe V-type proton ATPase subunit F (vma7) CSB-YP002407SXV
CSB-EP002407SXV
CSB-BP002407SXV
CSB-MP002407SXV
CSB-EP002407SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Ubiquitin-like modifier-activating enzyme atg7 (atg7), partial CSB-YP002294SXV
CSB-EP002294SXV
CSB-BP002294SXV
CSB-MP002294SXV
CSB-EP002294SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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