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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila yakuba Triosephosphate isomerase (Tpi) CSB-YP024102DMR
CSB-EP024102DMR
CSB-BP024102DMR
CSB-MP024102DMR
CSB-EP024102DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fibulin-1 (fbl-1), partial CSB-YP008452CXY
CSB-EP008452CXY
CSB-BP008452CXY
CSB-MP008452CXY
CSB-EP008452CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amblysomus hottentotus Aquaporin-2 (AQP2), partial CSB-YP001962BYB1
CSB-EP001962BYB1
CSB-BP001962BYB1
CSB-MP001962BYB1
CSB-EP001962BYB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dugong dugon Aquaporin-2 (AQP2), partial CSB-YP001962DMV1
CSB-EP001962DMV1
CSB-BP001962DMV1
CSB-MP001962DMV1
CSB-EP001962DMV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Echinops telfairi Alpha-2B adrenergic receptor (ADRA2B), partial CSB-YP001389EJK1
CSB-EP001389EJK1
CSB-BP001389EJK1
CSB-MP001389EJK1
CSB-EP001389EJK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichosurus vulpecula Inhibin alpha chain (INHA) CSB-YP011718TQG
CSB-EP011718TQG
CSB-BP011718TQG
CSB-MP011718TQG
CSB-EP011718TQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus ATP synthase protein 8 (mt-atp8), partial CSB-YP015071DQG
CSB-EP015071DQG
CSB-BP015071DQG
CSB-MP015071DQG
CSB-EP015071DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Cytochrome b (mt-cyb), partial CSB-YP015075DQG
CSB-EP015075DQG
CSB-BP015075DQG
CSB-MP015075DQG
CSB-EP015075DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nycticebus coucang NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076NIC
CSB-EP015076NIC
CSB-BP015076NIC
CSB-MP015076NIC
CSB-EP015076NIC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradypus variegatus NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076BVV
CSB-EP015076BVV
CSB-BP015076BVV
CSB-MP015076BVV
CSB-EP015076BVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phascolarctos cinereus NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076EUM
CSB-EP015076EUM
CSB-BP015076EUM
CSB-MP015076EUM
CSB-EP015076EUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sminthopsis macroura NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076SZU
CSB-EP015076SZU
CSB-BP015076SZU
CSB-MP015076SZU
CSB-EP015076SZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rupicapra pyrenaica Cytochrome b (MT-CYB), partial CSB-YP015075ROU
CSB-EP015075ROU
CSB-BP015075ROU
CSB-MP015075ROU
CSB-EP015075ROU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudois nayaur Cytochrome b (MT-CYB), partial CSB-YP015075EZV
CSB-EP015075EZV
CSB-BP015075EZV
CSB-MP015075EZV
CSB-EP015075EZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capra nubiana Cytochrome b (MT-CYB), partial CSB-YP015075DPZ
CSB-EP015075DPZ
CSB-BP015075DPZ
CSB-MP015075DPZ
CSB-EP015075DPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Struthio camelus NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078FOU1
CSB-EP015078FOU1
CSB-BP015078FOU1
CSB-MP015078FOU1
CSB-EP015078FOU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aptenodytes patagonicus Cytochrome b (MT-CYB), partial CSB-YP015075DNS
CSB-EP015075DNS
CSB-BP015075DNS
CSB-MP015075DNS
CSB-EP015075DNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fregetta tropica Cytochrome b (MT-CYB), partial CSB-YP015075FFK
CSB-EP015075FFK
CSB-BP015075FFK
CSB-MP015075FFK
CSB-EP015075FFK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrobates pelagicus Cytochrome b (MT-CYB), partial CSB-YP015075HXR
CSB-EP015075HXR
CSB-BP015075HXR
CSB-MP015075HXR
CSB-EP015075HXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanodroma furcata Cytochrome b (MT-CYB), partial CSB-YP015075ODQ
CSB-EP015075ODQ
CSB-BP015075ODQ
CSB-MP015075ODQ
CSB-EP015075ODQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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