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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Trans-cinnamate 4-monooxygenase (CYP73A5), partial CSB-YP310105DOA
CSB-EP310105DOA
CSB-BP310105DOA
CSB-MP310105DOA
CSB-EP310105DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein TERMINAL FLOWER 1 (TFL1) CSB-YP310106DOA
CSB-EP310106DOA
CSB-BP310106DOA
CSB-MP310106DOA
CSB-EP310106DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ubiquitin-activating enzyme E1 1 (UBA1), partial CSB-YP310107DOA
CSB-EP310107DOA
CSB-BP310107DOA
CSB-MP310107DOA
CSB-EP310107DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryococcus braunii L-lactate dehydrogenase CSB-YP310108BIZ
CSB-EP310108BIZ
CSB-BP310108BIZ
CSB-MP310108BIZ
CSB-EP310108BIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Flagellar WD repeat-containing protein Pf20 (PF20), partial CSB-YP310109DSJ
CSB-EP310109DSJ
CSB-BP310109DSJ
CSB-MP310109DSJ
CSB-EP310109DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fragaria ananassa Metallothionein-like protein type 2 MET1 (MET1) CSB-YP310110FEZ
CSB-EP310110FEZ
CSB-BP310110FEZ
CSB-MP310110FEZ
CSB-EP310110FEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum 14-3-3 protein 1 (TFT1) CSB-YP310111SZY
CSB-EP310111SZY
CSB-BP310111SZY
CSB-MP310111SZY
CSB-EP310111SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesembryanthemum crystallinum Ras-related protein Rab7A CSB-YP310112MRC
CSB-EP310112MRC
CSB-BP310112MRC
CSB-MP310112MRC
CSB-EP310112MRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00150 (AtMg00150), partial CSB-YP310113DOA1
CSB-EP310113DOA1
CSB-BP310113DOA1
CSB-MP310113DOA1
CSB-EP310113DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00440/AtMg01140 (AtMg00440) CSB-YP310114DOA
CSB-EP310114DOA
CSB-BP310114DOA
CSB-MP310114DOA
CSB-EP310114DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00530 (AtMg00530) CSB-YP310115DOA
CSB-EP310115DOA
CSB-BP310115DOA
CSB-MP310115DOA
CSB-EP310115DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00630 (AtMg00630) CSB-YP310116DOA
CSB-EP310116DOA
CSB-BP310116DOA
CSB-MP310116DOA
CSB-EP310116DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana plumbaginifolia NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN) CSB-YP310117NHC
CSB-EP310117NHC
CSB-BP310117NHC
CSB-MP310117NHC
CSB-EP310117NHC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum 14-3-3-like protein C CSB-YP310118NHE
CSB-EP310118NHE
CSB-BP310118NHE
CSB-MP310118NHE
CSB-EP310118NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Actin-66 CSB-YP310119NHE
CSB-EP310119NHE
CSB-BP310119NHE
CSB-MP310119NHE
CSB-EP310119NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Guanine nucleotide-binding protein subunit beta-2 CSB-YP310120NHE
CSB-EP310120NHE
CSB-BP310120NHE
CSB-MP310120NHE
CSB-EP310120NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Vacuolar-sorting receptor 1 (BP80), partial CSB-YP310121EWE
CSB-EP310121EWE
CSB-BP310121EWE
CSB-MP310121EWE
CSB-EP310121EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Actin-46 CSB-YP310123FIG
CSB-EP310123FIG
CSB-BP310123FIG
CSB-MP310123FIG
CSB-EP310123FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Preprotein translocase subunit SECY, chloroplastic (SECY), partial CSB-YP310124FKI
CSB-EP310124FKI
CSB-BP310124FKI
CSB-MP310124FKI
CSB-EP310124FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus kitakamiensis Caffeoyl-CoA O-methyltransferase CSB-YP310125PPS
CSB-EP310125PPS
CSB-BP310125PPS
CSB-MP310125PPS
CSB-EP310125PPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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