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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Germin-like protein subfamily 2 member 3 (GLP8) CSB-YP310509DOA
CSB-EP310509DOA
CSB-BP310509DOA
CSB-MP310509DOA
CSB-EP310509DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 30S ribosomal protein S5, chloroplastic (rps5), partial CSB-YP310510DOA
CSB-EP310510DOA
CSB-BP310510DOA
CSB-MP310510DOA
CSB-EP310510DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Isocitrate dehydrogenase [NAD] regulatory subunit 2, mitochondrial (IDH2) CSB-YP310511DOA
CSB-EP310511DOA
CSB-BP310511DOA
CSB-MP310511DOA
CSB-EP310511DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable LRR receptor-like serine/threonine-protein kinase RKF3 (RKF3), partial CSB-YP310512DOA
CSB-EP310512DOA
CSB-BP310512DOA
CSB-MP310512DOA
CSB-EP310512DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Casuarina glauca Acyl carrier protein 1, chloroplastic (ACP1) CSB-YP310513CEP
CSB-EP310513CEP
CSB-BP310513CEP
CSB-MP310513CEP
CSB-EP310513CEP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum 14-3-3 protein 3 (TFT3) CSB-YP310514SZY
CSB-EP310514SZY
CSB-BP310514SZY
CSB-MP310514SZY
CSB-EP310514SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Non-specific lipid-transfer protein 2 (LE16) CSB-YP310515SZY
CSB-EP310515SZY
CSB-BP310515SZY
CSB-MP310515SZY
CSB-EP310515SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesembryanthemum crystallinum Probable mannitol dehydrogenase (ELI3) CSB-YP310516MRC
CSB-EP310516MRC
CSB-BP310516MRC
CSB-MP310516MRC
CSB-EP310516MRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00010 (AtMg00010) CSB-YP310517DOA
CSB-EP310517DOA
CSB-BP310517DOA
CSB-MP310517DOA
CSB-EP310517DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative uncharacterized mitochondrial protein AtMg00280 (AtMg00280) CSB-YP310518DOA
CSB-EP310518DOA
CSB-BP310518DOA
CSB-MP310518DOA
CSB-EP310518DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00430/AtMg01150 (AtMg00430) CSB-YP310519DOA
CSB-EP310519DOA
CSB-BP310519DOA
CSB-MP310519DOA
CSB-EP310519DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Medicago truncatula Early nodulin-16 (ENOD16) CSB-YP310520MQP
CSB-EP310520MQP
CSB-BP310520MQP
CSB-MP310520MQP
CSB-EP310520MQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum 14-3-3-like protein A CSB-YP310521NHE
CSB-EP310521NHE
CSB-BP310521NHE
CSB-MP310521NHE
CSB-EP310521NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Histone H2B (HIS2B) CSB-YP310522NHE
CSB-EP310522NHE
CSB-BP310522NHE
CSB-MP310522NHE
CSB-EP310522NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Histidine--tRNA ligase (Os05g0150900, LOC_Os05g05840), partial CSB-YP310523OFG
CSB-EP310523OFG
CSB-BP310523OFG
CSB-MP310523OFG
CSB-EP310523OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Soluble starch synthase 1, chloroplastic/amyloplastic, partial CSB-YP310524FIG
CSB-EP310524FIG
CSB-BP310524FIG
CSB-MP310524FIG
CSB-EP310524FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Obtusifoliol 14-alpha demethylase (CYP51) CSB-YP310525TQN
CSB-EP310525TQN
CSB-BP310525TQN
CSB-MP310525TQN
CSB-EP310525TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phospholipase D beta 1 (PLDBETA1), partial CSB-YP310526DOA
CSB-EP310526DOA
CSB-BP310526DOA
CSB-MP310526DOA
CSB-EP310526DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable protein kinase At2g41970 (At2g41970) CSB-YP310527DOA
CSB-EP310527DOA
CSB-BP310527DOA
CSB-MP310527DOA
CSB-EP310527DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Calcium-dependent protein kinase 14 (CPK14), partial CSB-YP310528DOA
CSB-EP310528DOA
CSB-BP310528DOA
CSB-MP310528DOA
CSB-EP310528DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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