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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Gibbon hepatitis B virus subtype ayw3q External core antigen (C) CSB-YP310469GCB
CSB-EP310469GCB
CSB-BP310469GCB
CSB-MP310469GCB
CSB-EP310469GCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barley mild mosaic virus Genome polyprotein 1 CSB-YP310470BEL
CSB-EP310470BEL
CSB-BP310470BEL
CSB-MP310470BEL
CSB-EP310470BEL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Myosin heavy chain kinase B (mhkB), partial CSB-YP310471DKK
CSB-EP310471DKK
CSB-BP310471DKK
CSB-MP310471DKK
CSB-EP310471DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cation-transporting ATPase C10C6.6 (C10C6.6), partial CSB-YP310472CXY
CSB-EP310472CXY
CSB-BP310472CXY
CSB-MP310472CXY
CSB-EP310472CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable glycerol-3-phosphate dehydrogenase, mitochondrial (T25G3.4), partial CSB-YP310473CXY
CSB-EP310473CXY
CSB-BP310473CXY
CSB-MP310473CXY
CSB-EP310473CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized peptidase C1-like protein F26E4.3 (F26E4.3) CSB-YP310474CXY
CSB-EP310474CXY
CSB-BP310474CXY
CSB-MP310474CXY
CSB-EP310474CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein humpback-1 (hmp-1), partial CSB-YP310475CXY
CSB-EP310475CXY
CSB-BP310475CXY
CSB-MP310475CXY
CSB-EP310475CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylosuccinate synthetase (C37H5.6) CSB-YP310476CXY
CSB-EP310476CXY
CSB-BP310476CXY
CSB-MP310476CXY
CSB-EP310476CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Signal recognition particle 72 kDa protein homolog (F08D12.1), partial CSB-YP310477CXY
CSB-EP310477CXY
CSB-BP310477CXY
CSB-MP310477CXY
CSB-EP310477CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable glutathione S-transferase 7 (gst-7) CSB-YP310478CXY
CSB-EP310478CXY
CSB-BP310478CXY
CSB-MP310478CXY
CSB-EP310478CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome biogenesis protein WDR12 homolog (tag-345) CSB-YP310479CXY
CSB-EP310479CXY
CSB-BP310479CXY
CSB-MP310479CXY
CSB-EP310479CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serpentine receptor class H-72 (srh-72), partial CSB-YP310480CXY1
CSB-EP310480CXY1
CSB-BP310480CXY1
CSB-MP310480CXY1
CSB-EP310480CXY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila insularis Period circadian protein (per) CSB-YP310481DGE
CSB-EP310481DGE
CSB-BP310481DGE
CSB-MP310481DGE
CSB-EP310481DGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Probable multidrug resistance-associated protein lethal (2)03659, partial CSB-YP310482DLU
CSB-EP310482DLU
CSB-BP310482DLU
CSB-MP310482DLU
CSB-EP310482DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila pavlovskiana Period circadian protein (per) CSB-YP310483DGQ
CSB-EP310483DGQ
CSB-BP310483DGQ
CSB-MP310483DGQ
CSB-EP310483DGQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sucinea Period circadian protein (per) CSB-YP310484DGV
CSB-EP310484DGV
CSB-BP310484DGV
CSB-MP310484DGV
CSB-EP310484DGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymnaea stagnalis Fructose-bisphosphate aldolase CSB-YP310485LRQ
CSB-EP310485LRQ
CSB-BP310485LRQ
CSB-MP310485LRQ
CSB-EP310485LRQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma japonicum 25 kDa integral membrane protein, partial CSB-YP310486SXP1
CSB-EP310486SXP1
CSB-BP310486SXP1
CSB-MP310486SXP1
CSB-EP310486SXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spindle-defective protein 2 (spd-2), partial CSB-YP310487CXY
CSB-EP310487CXY
CSB-BP310487CXY
CSB-MP310487CXY
CSB-EP310487CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sepia officinalis FMRFamide-related neuropeptides (FMRFa) CSB-YP310488SYJ
CSB-EP310488SYJ
CSB-BP310488SYJ
CSB-MP310488SYJ
CSB-EP310488SYJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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