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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Uncharacterized ABC transporter solute-binding protein yclQ (yclQ) CSB-YP309414BRJ
CSB-EP309414BRJ
CSB-BP309414BRJ
CSB-MP309414BRJ
CSB-EP309414BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Fructose-bisphosphate aldolase (fba) CSB-YP309415GFM
CSB-EP309415GFM
CSB-BP309415GFM
CSB-MP309415GFM
CSB-EP309415GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis GTPase HflX (hflX) CSB-YP309416BRJ
CSB-EP309416BRJ
CSB-BP309416BRJ
CSB-MP309416BRJ
CSB-EP309416BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP309417BRJ
CSB-EP309417BRJ
CSB-BP309417BRJ
CSB-MP309417BRJ
CSB-EP309417BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Undecaprenyl-diphosphatase (uppP), partial CSB-YP309418BRJ1
CSB-EP309418BRJ1
CSB-BP309418BRJ1
CSB-MP309418BRJ1
CSB-EP309418BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Ribulokinase (araB), partial CSB-YP309419BRJ
CSB-EP309419BRJ
CSB-BP309419BRJ
CSB-MP309419BRJ
CSB-EP309419BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Carbon starvation protein A homolog (cstA), partial CSB-YP309420BRJ
CSB-EP309420BRJ
CSB-BP309420BRJ
CSB-MP309420BRJ
CSB-EP309420BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywoB (ywoB), partial CSB-YP309422BRJ1
CSB-EP309422BRJ1
CSB-BP309422BRJ1
CSB-MP309422BRJ1
CSB-EP309422BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ywoH (ywoH) CSB-YP309423BRJ
CSB-EP309423BRJ
CSB-BP309423BRJ
CSB-MP309423BRJ
CSB-EP309423BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Large-conductance mechanosensitive channel (mscL), partial CSB-YP309424BRJ
CSB-EP309424BRJ
CSB-BP309424BRJ
CSB-MP309424BRJ
CSB-EP309424BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Glutamate dehydrogenase (gdhA) CSB-YP309425BDU
CSB-EP309425BDU
CSB-BP309425BDU
CSB-MP309425BDU
CSB-EP309425BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii tRNA modification GTPase MnmE (mnmE) CSB-YP309426DXP
CSB-EP309426DXP
CSB-BP309426DXP
CSB-MP309426DXP
CSB-EP309426DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Lysine exporter protein (lysE), partial CSB-YP309427DWY1
CSB-EP309427DWY1
CSB-BP309427DWY1
CSB-MP309427DWY1
CSB-EP309427DWY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter agglomerans Mercuric transport protein (merT), partial CSB-YP309428EKP1
CSB-EP309428EKP1
CSB-BP309428EKP1
CSB-MP309428EKP1
CSB-EP309428EKP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL), partial CSB-YP309429HHO
CSB-EP309429HHO
CSB-BP309429HHO
CSB-MP309429HHO
CSB-EP309429HHO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula vallismortis Cruxhalorhodopsin-3 (choP3), partial CSB-YP309430HBE1
CSB-EP309430HBE1
CSB-BP309430HBE1
CSB-MP309430HBE1
CSB-EP309430HBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Glutamate 5-kinase (proB) CSB-YP309431LOS
CSB-EP309431LOS
CSB-BP309431LOS
CSB-MP309431LOS
CSB-EP309431LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina barkeri Methenyltetrahydromethanopterin cyclohydrolase (mch) CSB-YP309432MSM
CSB-EP309432MSM
CSB-BP309432MSM
CSB-MP309432MSM
CSB-EP309432MSM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanopyrus kandleri F420-dependent methylenetetrahydromethanopterin dehydrogenase (mtd) CSB-YP309433MSI
CSB-EP309433MSI
CSB-BP309433MSI
CSB-MP309433MSI
CSB-EP309433MSI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP309434MVZ
CSB-EP309434MVZ
CSB-BP309434MVZ
CSB-MP309434MVZ
CSB-EP309434MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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