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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanococcus vannielii Flagellin B1 (flaB1) CSB-YP311357MNS
CSB-EP311357MNS
CSB-BP311357MNS
CSB-MP311357MNS
CSB-EP311357MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative arsenate reductase (arsC) CSB-YP311358NFZ
CSB-EP311358NFZ
CSB-BP311358NFZ
CSB-MP311358NFZ
CSB-EP311358NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein nirG (nirG) CSB-YP311360EZX
CSB-EP311360EZX
CSB-BP311360EZX
CSB-MP311360EZX
CSB-EP311360EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Probable RNA-binding protein PA4753 (PA4753) CSB-YP311361EZX
CSB-EP311361EZX
CSB-BP311361EZX
CSB-MP311361EZX
CSB-EP311361EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aromatic-amino-acid aminotransferase (tyrB) CSB-YP311362PBZ
CSB-EP311362PBZ
CSB-BP311362PBZ
CSB-MP311362PBZ
CSB-EP311362PBZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorophyll a/b light-harvesting protein pcbB (pcbB), partial CSB-YP311363PSD1
CSB-EP311363PSD1
CSB-BP311363PSD1
CSB-MP311363PSD1
CSB-EP311363PSD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Protein RecA (recA) CSB-YP311364ESG
CSB-EP311364ESG
CSB-BP311364ESG
CSB-MP311364ESG
CSB-EP311364ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus kodakaraensis DNA repair and recombination protein radB (radB) CSB-YP311365FHY
CSB-EP311365FHY
CSB-BP311365FHY
CSB-MP311365FHY
CSB-EP311365FHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Bifunctional protein putA (putA), partial CSB-YP311366RKT
CSB-EP311366RKT
CSB-BP311366RKT
CSB-MP311366RKT
CSB-EP311366RKT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Protein CbbX (cbbX) CSB-YP311367RLE
CSB-EP311367RLE
CSB-BP311367RLE
CSB-MP311367RLE
CSB-EP311367RLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus acidocaldarius Probable translation initiation factor IF-2 (infB), partial CSB-YP311368FOZ
CSB-EP311368FOZ
CSB-BP311368FOZ
CSB-MP311368FOZ
CSB-EP311368FOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chain length determinant protein (wzzB), partial CSB-YP311369SZA
CSB-EP311369SZA
CSB-BP311369SZA
CSB-MP311369SZA
CSB-EP311369SZA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 8-oxo-dGTP diphosphatase (mutX) CSB-YP311370FMR
CSB-EP311370FMR
CSB-BP311370FMR
CSB-MP311370FMR
CSB-EP311370FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaJ (dnaJ) CSB-YP311371FMW
CSB-EP311371FMW
CSB-BP311371FMW
CSB-MP311371FMW
CSB-EP311371FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Uncharacterized protein SSO2105 (SSO2105) CSB-YP311372FPM
CSB-EP311372FPM
CSB-BP311372FPM
CSB-MP311372FPM
CSB-EP311372FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Uncharacterized ATP-dependent helicase SSO0112 (SSO0112), partial CSB-YP311373FPM
CSB-EP311373FPM
CSB-BP311373FPM
CSB-MP311373FPM
CSB-EP311373FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Methionine aminopeptidase (map) CSB-YP311374FPM
CSB-EP311374FPM
CSB-BP311374FPM
CSB-MP311374FPM
CSB-EP311374FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Tyrosine--tRNA ligase (tyrS) CSB-YP311375FPM
CSB-EP311375FPM
CSB-BP311375FPM
CSB-MP311375FPM
CSB-EP311375FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus UPF0103 protein SSO0066 (SSO0066) CSB-YP311376FPM
CSB-EP311376FPM
CSB-BP311376FPM
CSB-MP311376FPM
CSB-EP311376FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus 50S ribosomal protein L1P (rpl1p) CSB-YP311377FPM
CSB-EP311377FPM
CSB-BP311377FPM
CSB-MP311377FPM
CSB-EP311377FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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