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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thanatephorus cucumeris Laccase-4 (LCC4), partial CSB-YP311581TCE
CSB-EP311581TCE
CSB-BP311581TCE
CSB-MP311581TCE
CSB-EP311581TCE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Protein kinase rad3 (rad3), partial CSB-YP311582SXV
CSB-EP311582SXV
CSB-BP311582SXV
CSB-MP311582SXV
CSB-EP311582SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Membrane-bound protein lytA (lytA) CSB-YP311583BRJ
CSB-EP311583BRJ
CSB-BP311583BRJ
CSB-MP311583BRJ
CSB-EP311583BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP311584LNG
CSB-EP311584LNG
CSB-BP311584LNG
CSB-MP311584LNG
CSB-EP311584LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Acetolactate synthase small subunit (ilvH) CSB-YP311585LNG
CSB-EP311585LNG
CSB-BP311585LNG
CSB-MP311585LNG
CSB-EP311585LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP311586LNG
CSB-EP311586LNG
CSB-BP311586LNG
CSB-MP311586LNG
CSB-EP311586LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hexokinase (HK) CSB-YP311587PLO
CSB-EP311587PLO
CSB-BP311587PLO
CSB-MP311587PLO
CSB-EP311587PLO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African horse sickness virus 4 Outer capsid protein VP5 (S6), partial CSB-YP311588ADZ
CSB-EP311588ADZ
CSB-BP311588ADZ
CSB-MP311588ADZ
CSB-EP311588ADZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Bilin biosynthesis protein MpeV (mpeV) CSB-YP311589SSK
CSB-EP311589SSK
CSB-BP311589SSK
CSB-MP311589SSK
CSB-EP311589SSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Chromophore lyase CpcT/CpeT (cpcT) CSB-YP311590SSK
CSB-EP311590SSK
CSB-BP311590SSK
CSB-MP311590SSK
CSB-EP311590SSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae N-alpha-acetyltransferase, 35 NatC auxiliary subunit (MAK10), partial CSB-YP311591SVG
CSB-EP311591SVG
CSB-BP311591SVG
CSB-MP311591SVG
CSB-EP311591SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Cysteine--tRNA ligase (cysS) CSB-YP311592SIU
CSB-EP311592SIU
CSB-BP311592SIU
CSB-MP311592SIU
CSB-EP311592SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Topoisomerase 1-associated factor 2 (TOF2), partial CSB-YP311593SVG
CSB-EP311593SVG
CSB-BP311593SVG
CSB-MP311593SVG
CSB-EP311593SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida tropicalis Malate synthase, glyoxysomal (PMS1), partial CSB-YP311594CZN
CSB-EP311594CZN
CSB-BP311594CZN
CSB-MP311594CZN
CSB-EP311594CZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloferax mediterranei Protein gvpH (gvpH) CSB-YP311595HTS
CSB-EP311595HTS
CSB-BP311595HTS
CSB-MP311595HTS
CSB-EP311595HTS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pygmy chimpanzee papillomavirus type 1 Regulatory protein E2 (E2) CSB-YP311596PXM
CSB-EP311596PXM
CSB-BP311596PXM
CSB-MP311596PXM
CSB-EP311596PXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pygmy chimpanzee papillomavirus type 1 Protein E6 (E6) CSB-YP311597PXM
CSB-EP311597PXM
CSB-BP311597PXM
CSB-MP311597PXM
CSB-EP311597PXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Long-chain-fatty-acid--AMP ligase FadD28 (fadD28), partial CSB-YP311598MVH
CSB-EP311598MVH
CSB-BP311598MVH
CSB-MP311598MVH
CSB-EP311598MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter agglomerans P-protein (pheA) CSB-YP311599EKP
CSB-EP311599EKP
CSB-BP311599EKP
CSB-MP311599EKP
CSB-EP311599EKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Acetate kinase (ackA) CSB-YP311600SIU
CSB-EP311600SIU
CSB-BP311600SIU
CSB-MP311600SIU
CSB-EP311600SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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