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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus pneumoniae serotype 2 UPF0397 protein SPD_0432 (SPD_0432), partial CSB-YP312126FNF1
CSB-EP312126FNF1
CSB-BP312126FNF1
CSB-MP312126FNF1
CSB-EP312126FNF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Ketol-acid reductoisomerase (ilvC) CSB-YP312127FNF
CSB-EP312127FNF
CSB-BP312127FNF
CSB-MP312127FNF
CSB-EP312127FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 N utilization substance protein B homolog (nusB) CSB-YP312128FNF
CSB-EP312128FNF
CSB-BP312128FNF
CSB-MP312128FNF
CSB-EP312128FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 MutS2 protein (mutS2), partial CSB-YP312129FNF
CSB-EP312129FNF
CSB-BP312129FNF
CSB-MP312129FNF
CSB-EP312129FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Holliday junction resolvase recU (recU) CSB-YP312130FNF
CSB-EP312130FNF
CSB-BP312130FNF
CSB-MP312130FNF
CSB-EP312130FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Proline--tRNA ligase (proS), partial CSB-YP312131FNF
CSB-EP312131FNF
CSB-BP312131FNF
CSB-MP312131FNF
CSB-EP312131FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 50S ribosomal protein L15 (rplO) CSB-YP312132FNF
CSB-EP312132FNF
CSB-BP312132FNF
CSB-MP312132FNF
CSB-EP312132FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 30S ribosomal protein S14 (rpsN) CSB-YP312133FNF
CSB-EP312133FNF
CSB-BP312133FNF
CSB-MP312133FNF
CSB-EP312133FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 30S ribosomal protein S3 (rpsC) CSB-YP312134FNF
CSB-EP312134FNF
CSB-BP312134FNF
CSB-MP312134FNF
CSB-EP312134FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP312135FNF
CSB-EP312135FNF
CSB-BP312135FNF
CSB-MP312135FNF
CSB-EP312135FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 2 Phosphate acyltransferase (plsX) CSB-YP312136FNF
CSB-EP312136FNF
CSB-BP312136FNF
CSB-MP312136FNF
CSB-EP312136FNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Elongation factor P (efp) CSB-YP312137LES
CSB-EP312137LES
CSB-BP312137LES
CSB-MP312137LES
CSB-EP312137LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Protease HtpX homolog (htpX), partial CSB-YP312138LES1
CSB-EP312138LES1
CSB-BP312138LES1
CSB-MP312138LES1
CSB-EP312138LES1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Flagellar assembly factor FliW (fliW) CSB-YP312139LES
CSB-EP312139LES
CSB-BP312139LES
CSB-MP312139LES
CSB-EP312139LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP312140LES
CSB-EP312140LES
CSB-BP312140LES
CSB-MP312140LES
CSB-EP312140LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP312141LES
CSB-EP312141LES
CSB-BP312141LES
CSB-MP312141LES
CSB-EP312141LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 50S ribosomal protein L4 (rplD) CSB-YP312142LES
CSB-EP312142LES
CSB-BP312142LES
CSB-MP312142LES
CSB-EP312142LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 50S ribosomal protein L14 (rplN) CSB-YP312143LES
CSB-EP312143LES
CSB-BP312143LES
CSB-MP312143LES
CSB-EP312143LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 30S ribosomal protein S5 (rpsE) CSB-YP312144LES
CSB-EP312144LES
CSB-BP312144LES
CSB-MP312144LES
CSB-EP312144LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 50S ribosomal protein L36 (rpmJ) CSB-YP312145LES
CSB-EP312145LES
CSB-BP312145LES
CSB-MP312145LES
CSB-EP312145LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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