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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 30S ribosomal protein S4 (rpsD) CSB-YP312146LES
CSB-EP312146LES
CSB-BP312146LES
CSB-MP312146LES
CSB-EP312146LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 50S ribosomal protein L17 (rplQ) CSB-YP312147LES
CSB-EP312147LES
CSB-BP312147LES
CSB-MP312147LES
CSB-EP312147LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 50S ribosomal protein L27 (rpmA) CSB-YP312148LES
CSB-EP312148LES
CSB-BP312148LES
CSB-MP312148LES
CSB-EP312148LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Alanine--tRNA ligase (alaS), partial CSB-YP312149LES
CSB-EP312149LES
CSB-BP312149LES
CSB-MP312149LES
CSB-EP312149LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 50S ribosomal protein L10 (rplJ) CSB-YP312150LES
CSB-EP312150LES
CSB-BP312150LES
CSB-MP312150LES
CSB-EP312150LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Lipoprotein signal peptidase (lspA), partial CSB-YP312151LES1
CSB-EP312151LES1
CSB-BP312151LES1
CSB-MP312151LES1
CSB-EP312151LES1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP312153LES
CSB-EP312153LES
CSB-BP312153LES
CSB-MP312153LES
CSB-EP312153LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Homoserine O-acetyltransferase (metX) CSB-YP312154LES
CSB-EP312154LES
CSB-BP312154LES
CSB-MP312154LES
CSB-EP312154LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Biotin synthase (bioB) CSB-YP312155LES
CSB-EP312155LES
CSB-BP312155LES
CSB-MP312155LES
CSB-EP312155LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis ATP synthase epsilon chain (atpC) CSB-YP312156LES
CSB-EP312156LES
CSB-BP312156LES
CSB-MP312156LES
CSB-EP312156LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis S-adenosylmethionine synthase (metK) CSB-YP312157LES
CSB-EP312157LES
CSB-BP312157LES
CSB-MP312157LES
CSB-EP312157LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Protein translocase subunit SecA (secA), partial CSB-YP312158LES
CSB-EP312158LES
CSB-BP312158LES
CSB-MP312158LES
CSB-EP312158LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Putative arginyl-tRNA--protein transferase (ate) CSB-YP312159LES
CSB-EP312159LES
CSB-BP312159LES
CSB-MP312159LES
CSB-EP312159LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 30S ribosomal protein S21 (rpsU) CSB-YP312160LES
CSB-EP312160LES
CSB-BP312160LES
CSB-MP312160LES
CSB-EP312160LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Deoxyguanosinetriphosphate triphosphohydrolase-like protein (LBJ_1337) CSB-YP312161LES
CSB-EP312161LES
CSB-BP312161LES
CSB-MP312161LES
CSB-EP312161LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis UPF0235 protein LBJ_1240 (LBJ_1240) CSB-YP312162LES
CSB-EP312162LES
CSB-BP312162LES
CSB-MP312162LES
CSB-EP312162LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Trigger factor (tig) CSB-YP312163LES
CSB-EP312163LES
CSB-BP312163LES
CSB-MP312163LES
CSB-EP312163LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 30S ribosomal protein S15 (rpsO) CSB-YP312164LES
CSB-EP312164LES
CSB-BP312164LES
CSB-MP312164LES
CSB-EP312164LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 50S ribosomal protein L20 (rplT) CSB-YP312165LES
CSB-EP312165LES
CSB-BP312165LES
CSB-MP312165LES
CSB-EP312165LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis LL-diaminopimelate aminotransferase (dapL) CSB-YP312166LES
CSB-EP312166LES
CSB-BP312166LES
CSB-MP312166LES
CSB-EP312166LES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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