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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Lactobacillus casei Phosphopantetheine adenylyltransferase (coaD) CSB-YP312757LAN
CSB-EP312757LAN
CSB-BP312757LAN
CSB-MP312757LAN
CSB-EP312757LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Probable tRNA sulfurtransferase (thiI) CSB-YP312758LAN
CSB-EP312758LAN
CSB-BP312758LAN
CSB-MP312758LAN
CSB-EP312758LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP312759LAN
CSB-EP312759LAN
CSB-BP312759LAN
CSB-MP312759LAN
CSB-EP312759LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei UPF0735 ACT domain-containing protein LSEI_1046 (LSEI_1046) CSB-YP312760LAN
CSB-EP312760LAN
CSB-BP312760LAN
CSB-MP312760LAN
CSB-EP312760LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei UvrABC system protein B (uvrB), partial CSB-YP312761LAN
CSB-EP312761LAN
CSB-BP312761LAN
CSB-MP312761LAN
CSB-EP312761LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei GMP reductase (guaC) CSB-YP312762LAN
CSB-EP312762LAN
CSB-BP312762LAN
CSB-MP312762LAN
CSB-EP312762LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP312763LAN
CSB-EP312763LAN
CSB-BP312763LAN
CSB-MP312763LAN
CSB-EP312763LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 6-phospho-alpha-glucosidase 1 (simA) CSB-YP312764LAN
CSB-EP312764LAN
CSB-BP312764LAN
CSB-MP312764LAN
CSB-EP312764LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP312765LAN
CSB-EP312765LAN
CSB-BP312765LAN
CSB-MP312765LAN
CSB-EP312765LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Pyrrolidone-carboxylate peptidase (pcp) CSB-YP312766LAN
CSB-EP312766LAN
CSB-BP312766LAN
CSB-MP312766LAN
CSB-EP312766LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei N-acetyldiaminopimelate deacetylase (LSEI_0096) CSB-YP312767LAN
CSB-EP312767LAN
CSB-BP312767LAN
CSB-MP312767LAN
CSB-EP312767LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 30S ribosomal protein S6 (rpsF) CSB-YP312768LAN
CSB-EP312768LAN
CSB-BP312768LAN
CSB-MP312768LAN
CSB-EP312768LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pediococcus pentosaceus tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP312769PEE
CSB-EP312769PEE
CSB-BP312769PEE
CSB-MP312769PEE
CSB-EP312769PEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pediococcus pentosaceus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP312770PEE
CSB-EP312770PEE
CSB-BP312770PEE
CSB-MP312770PEE
CSB-EP312770PEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pediococcus pentosaceus Probable D-serine dehydratase (dsdA) CSB-YP312771PEE
CSB-EP312771PEE
CSB-BP312771PEE
CSB-MP312771PEE
CSB-EP312771PEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pediococcus pentosaceus DNA ligase (ligA), partial CSB-YP312772PEE
CSB-EP312772PEE
CSB-BP312772PEE
CSB-MP312772PEE
CSB-EP312772PEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pediococcus pentosaceus Alanine racemase (alr) CSB-YP312773PEE
CSB-EP312773PEE
CSB-BP312773PEE
CSB-MP312773PEE
CSB-EP312773PEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pediococcus pentosaceus Initiation-control protein yabA (PEPE_1478) CSB-YP312774PEE
CSB-EP312774PEE
CSB-BP312774PEE
CSB-MP312774PEE
CSB-EP312774PEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pediococcus pentosaceus 30S ribosomal protein S19 (rpsS) CSB-YP312775PEE
CSB-EP312775PEE
CSB-BP312775PEE
CSB-MP312775PEE
CSB-EP312775PEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pediococcus pentosaceus 50S ribosomal protein L6 (rplF) CSB-YP312776PEE
CSB-EP312776PEE
CSB-BP312776PEE
CSB-MP312776PEE
CSB-EP312776PEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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