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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila melanogaster Guanine nucleotide-binding protein G (f) subunit alpha CSB-YP312228DLU
CSB-EP312228DLU
CSB-BP312228DLU
CSB-MP312228DLU
CSB-EP312228DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fragaria ananassa Auxin-repressed 12.5 kDa protein CSB-YP312229FEZ
CSB-EP312229FEZ
CSB-BP312229FEZ
CSB-MP312229FEZ
CSB-EP312229FEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative polyketide hydroxylase (schC), partial CSB-YP312230SON
CSB-EP312230SON
CSB-BP312230SON
CSB-MP312230SON
CSB-EP312230SON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein schB (schB) CSB-YP312231SON
CSB-EP312231SON
CSB-BP312231SON
CSB-MP312231SON
CSB-EP312231SON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP synthase epsilon chain (atpC) CSB-YP312232SSI
CSB-EP312232SSI
CSB-BP312232SSI
CSB-MP312232SSI
CSB-EP312232SSI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Tryptophan synthase alpha chain (trpA) CSB-YP312233LET
CSB-EP312233LET
CSB-BP312233LET
CSB-MP312233LET
CSB-EP312233LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP312234LET
CSB-EP312234LET
CSB-BP312234LET
CSB-MP312234LET
CSB-EP312234LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Glutamate racemase (murI) CSB-YP312235LET
CSB-EP312235LET
CSB-BP312235LET
CSB-MP312235LET
CSB-EP312235LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L35 (rpmI) CSB-YP312236MLL
CSB-EP312236MLL
CSB-BP312236MLL
CSB-MP312236MLL
CSB-EP312236MLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Virulence-associated protein vagC (vagC) CSB-YP312237SWM
CSB-EP312237SWM
CSB-BP312237SWM
CSB-MP312237SWM
CSB-EP312237SWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YDR333C (YDR333C), partial CSB-YP312238SVG
CSB-EP312238SVG
CSB-BP312238SVG
CSB-MP312238SVG
CSB-EP312238SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Camelpox virus A-type inclusion protein, partial CSB-YP312239CBS
CSB-EP312239CBS
CSB-BP312239CBS
CSB-MP312239CBS
CSB-EP312239CBS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia glumae Lipase chaperone (lifO), partial CSB-YP312240BNR1
CSB-EP312240BNR1
CSB-BP312240BNR1
CSB-MP312240BNR1
CSB-EP312240BNR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis B virus genotype F2 Large envelope protein (S), partial CSB-YP312241HEP1
CSB-EP312241HEP1
CSB-BP312241HEP1
CSB-MP312241HEP1
CSB-EP312241HEP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Shikimate dehydrogenase (aroE) CSB-YP312242LET
CSB-EP312242LET
CSB-BP312242LET
CSB-MP312242LET
CSB-EP312242LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 50S ribosomal protein L11 (rplK) CSB-YP312243LET
CSB-EP312243LET
CSB-BP312243LET
CSB-MP312243LET
CSB-EP312243LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis UPF0176 protein LBL_0674 (LBL_0674) CSB-YP312244LET
CSB-EP312244LET
CSB-BP312244LET
CSB-MP312244LET
CSB-EP312244LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 50S ribosomal protein L27 (rpmA) CSB-YP312245LET
CSB-EP312245LET
CSB-BP312245LET
CSB-MP312245LET
CSB-EP312245LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Leucine--tRNA ligase (leuS), partial CSB-YP312246LET
CSB-EP312246LET
CSB-BP312246LET
CSB-MP312246LET
CSB-EP312246LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dickeya dadantii 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (kduD) CSB-YP312247DKD
CSB-EP312247DKD
CSB-BP312247DKD
CSB-MP312247DKD
CSB-EP312247DKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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