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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Buchnera aphidicola subsp. Cinara cedri 50S ribosomal protein L35 (rpmI) CSB-YP312288BNA
CSB-EP312288BNA
CSB-BP312288BNA
CSB-MP312288BNA
CSB-EP312288BNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Sulfite reductase, assimilatory-type (DVU_1597) CSB-YP312289DDH
CSB-EP312289DDH
CSB-BP312289DDH
CSB-MP312289DDH
CSB-EP312289DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pedobacter heparinus Heparin lyase I CSB-YP312290PEF
CSB-EP312290PEF
CSB-BP312290PEF
CSB-MP312290PEF
CSB-EP312290PEF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mytilus trossulus Sperm-specific protein PHI-2B/PHI-3 CSB-YP312291MMR
CSB-EP312291MMR
CSB-BP312291MMR
CSB-MP312291MMR
CSB-EP312291MMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized peptide chain release factor-like protein YLR281C, mitochondrial (YLR281C) CSB-YP312292SVG
CSB-EP312292SVG
CSB-BP312292SVG
CSB-MP312292SVG
CSB-EP312292SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Metallothionein (mt-a) CSB-YP312293XBE
CSB-EP312293XBE
CSB-BP312293XBE
CSB-MP312293XBE
CSB-EP312293XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Cinara cedri Histidinol-phosphate aminotransferase (hisC) CSB-YP312294BNA
CSB-EP312294BNA
CSB-BP312294BNA
CSB-MP312294BNA
CSB-EP312294BNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Cinara cedri 50S ribosomal protein L33 (rpmG) CSB-YP312295BNA
CSB-EP312295BNA
CSB-BP312295BNA
CSB-MP312295BNA
CSB-EP312295BNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Cinara cedri DNA ligase (ligA), partial CSB-YP312296BNA
CSB-EP312296BNA
CSB-BP312296BNA
CSB-MP312296BNA
CSB-EP312296BNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Cinara cedri 30S ribosomal protein S21 (rpsU) CSB-YP312297BNA
CSB-EP312297BNA
CSB-BP312297BNA
CSB-MP312297BNA
CSB-EP312297BNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Cinara cedri DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP312298BNA
CSB-EP312298BNA
CSB-BP312298BNA
CSB-MP312298BNA
CSB-EP312298BNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Cinara cedri tRNA modification GTPase MnmE (mnmE) CSB-YP312299BNA
CSB-EP312299BNA
CSB-BP312299BNA
CSB-MP312299BNA
CSB-EP312299BNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Magnesium transporter MRS2-11, chloroplastic (MRS2-11), partial CSB-YP312300DOA1
CSB-EP312300DOA1
CSB-BP312300DOA1
CSB-MP312300DOA1
CSB-EP312300DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar import and degradation protein 22 (VID22), partial CSB-YP312301SVG
CSB-EP312301SVG
CSB-BP312301SVG
CSB-MP312301SVG
CSB-EP312301SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Zinc finger protein STP3 (STP3) CSB-YP312302SVG
CSB-EP312302SVG
CSB-BP312302SVG
CSB-MP312302SVG
CSB-EP312302SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydra oligactis Heat shock 70 kDa protein (HSP70) CSB-YP312303HQU
CSB-EP312303HQU
CSB-BP312303HQU
CSB-MP312303HQU
CSB-EP312303HQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loligo bleekeri Sodium channel protein 1 brain, partial CSB-YP312304LQH
CSB-EP312304LQH
CSB-BP312304LQH
CSB-MP312304LQH
CSB-EP312304LQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Chaperone protein DnaJ (dnaJ) CSB-YP312305BPI
CSB-EP312305BPI
CSB-BP312305BPI
CSB-MP312305BPI
CSB-EP312305BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Convolvulus tricolor Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP312306CPO
CSB-EP312306CPO
CSB-BP312306CPO
CSB-MP312306CPO
CSB-EP312306CPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Symphoricarpos albus Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP312307SRZ
CSB-EP312307SRZ
CSB-BP312307SRZ
CSB-MP312307SRZ
CSB-EP312307SRZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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