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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae LETM1 domain-containing protein YLH47, mitochondrial (YLH47), partial CSB-YP312370SVG1
CSB-EP312370SVG1
CSB-BP312370SVG1
CSB-MP312370SVG1
CSB-EP312370SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae N-terminal acetyltransferase B complex catalytic subunit NAT3 (NAT3) CSB-YP312371SVG
CSB-EP312371SVG
CSB-BP312371SVG
CSB-MP312371SVG
CSB-EP312371SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Caffeic acid 3-O-methyltransferase CSB-YP312372ZAX
CSB-EP312372ZAX
CSB-BP312372ZAX
CSB-MP312372ZAX
CSB-EP312372ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor URN1 (URN1) CSB-YP312373SVG
CSB-EP312373SVG
CSB-BP312373SVG
CSB-MP312373SVG
CSB-EP312373SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YPR153W (YPR153W), partial CSB-YP312374SVG1
CSB-EP312374SVG1
CSB-BP312374SVG1
CSB-MP312374SVG1
CSB-EP312374SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae GPN-loop GTPase 3 homolog YLR243W (YLR243W) CSB-YP312375SVG
CSB-EP312375SVG
CSB-BP312375SVG
CSB-MP312375SVG
CSB-EP312375SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum Exoenzymes regulatory protein AepA (aepA) CSB-YP312376ESP
CSB-EP312376ESP
CSB-BP312376ESP
CSB-MP312376ESP
CSB-EP312376ESP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Myosin light chain 2 (MLC2) CSB-YP312377SVG
CSB-EP312377SVG
CSB-BP312377SVG
CSB-MP312377SVG
CSB-EP312377SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratitis capitata Cecropin-1 (CEC1) CSB-YP312378DRF
CSB-EP312378DRF
CSB-BP312378DRF
CSB-MP312378DRF
CSB-EP312378DRF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Maltose fermentation regulatory protein YPR196W (YPR196W) CSB-YP312379SVG
CSB-EP312379SVG
CSB-BP312379SVG
CSB-MP312379SVG
CSB-EP312379SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera Apidaecins type 14 (APID14) CSB-YP312380DNK
CSB-EP312380DNK
CSB-BP312380DNK
CSB-MP312380DNK
CSB-EP312380DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nuclear envelope protein YPR174C (YPR174C) CSB-YP312381SVG
CSB-EP312381SVG
CSB-BP312381SVG
CSB-MP312381SVG
CSB-EP312381SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-lactamase (bla) CSB-YP312382SNV
CSB-EP312382SNV
CSB-BP312382SNV
CSB-MP312382SNV
CSB-EP312382SNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato mottle virus Nuclear shuttle protein (BR1, BV1) CSB-YP312383TGL
CSB-EP312383TGL
CSB-BP312383TGL
CSB-MP312383TGL
CSB-EP312383TGL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autographa californica nuclear polyhedrosis virus Uncharacterized 15.5 kDa protein in IAP2-VLF1 intergenic region CSB-YP312384ARA
CSB-EP312384ARA
CSB-BP312384ARA
CSB-MP312384ARA
CSB-EP312384ARA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae FIT family protein YFT2 (YFT2), partial CSB-YP312385SVG1
CSB-EP312385SVG1
CSB-BP312385SVG1
CSB-MP312385SVG1
CSB-EP312385SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Copper transport protein CTR3 (CTR3), partial CSB-YP312386SVG1
CSB-EP312386SVG1
CSB-BP312386SVG1
CSB-MP312386SVG1
CSB-EP312386SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autographa californica nuclear polyhedrosis virus Uncharacterized 12.5 kDa protein in VLF1-GP41 intergenic region CSB-YP312387ARA
CSB-EP312387ARA
CSB-BP312387ARA
CSB-MP312387ARA
CSB-EP312387ARA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative ATP-dependent RNA helicase YLR419W (YLR419W), partial CSB-YP312388SVG
CSB-EP312388SVG
CSB-BP312388SVG
CSB-MP312388SVG
CSB-EP312388SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Golgin IMH1 (IMH1), partial CSB-YP312389SVG
CSB-EP312389SVG
CSB-BP312389SVG
CSB-MP312389SVG
CSB-EP312389SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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