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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Glycine max Early nodulin-55-1 (ENOD55-1) CSB-YP312248GGV
CSB-EP312248GGV
CSB-BP312248GGV
CSB-MP312248GGV
CSB-EP312248GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maltose-binding periplasmic protein (malE) CSB-YP312249KBG
CSB-EP312249KBG
CSB-BP312249KBG
CSB-MP312249KBG
CSB-EP312249KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavaminate synthase 1 (cs1) CSB-YP312250FOA
CSB-EP312250FOA
CSB-BP312250FOA
CSB-MP312250FOA
CSB-EP312250FOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Regulatory protein pocR (pocR) CSB-YP312251SXB
CSB-EP312251SXB
CSB-BP312251SXB
CSB-MP312251SXB
CSB-EP312251SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Cobyric acid synthase (cbiP), partial CSB-YP312252SXB
CSB-EP312252SXB
CSB-BP312252SXB
CSB-MP312252SXB
CSB-EP312252SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 30S ribosomal protein S13 (rpsM1) CSB-YP312253LET
CSB-EP312253LET
CSB-BP312253LET
CSB-MP312253LET
CSB-EP312253LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 50S ribosomal protein L30 (rpmD1) CSB-YP312254LET
CSB-EP312254LET
CSB-BP312254LET
CSB-MP312254LET
CSB-EP312254LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis 50S ribosomal protein L2 (rplB1) CSB-YP312255LET
CSB-EP312255LET
CSB-BP312255LET
CSB-MP312255LET
CSB-EP312255LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Biopolymer transport protein exbB (exbB), partial CSB-YP312256FFZ1
CSB-EP312256FFZ1
CSB-BP312256FFZ1
CSB-MP312256FFZ1
CSB-EP312256FFZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Bypass of stop codon protein 2 (BSC2), partial CSB-YP312257SVG1
CSB-EP312257SVG1
CSB-BP312257SVG1
CSB-MP312257SVG1
CSB-EP312257SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Dihydropteroate synthase (Cbei_0206) CSB-YP312258DUC
CSB-EP312258DUC
CSB-BP312258DUC
CSB-MP312258DUC
CSB-EP312258DUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Protein CbiG (cbiG) CSB-YP312259SXB
CSB-EP312259SXB
CSB-BP312259SXB
CSB-MP312259SXB
CSB-EP312259SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces olivaceoviridis Exochitinase 1 (chi01), partial CSB-YP312260SPB
CSB-EP312260SPB
CSB-BP312260SPB
CSB-MP312260SPB
CSB-EP312260SPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaJ (dnaJ) CSB-YP312261EGM
CSB-EP312261EGM
CSB-BP312261EGM
CSB-MP312261EGM
CSB-EP312261EGM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YDR282C (YDR282C), partial CSB-YP312262SVG1
CSB-EP312262SVG1
CSB-BP312262SVG1
CSB-MP312262SVG1
CSB-EP312262SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cauliflower mosaic virus Transactivator/viroplasmin protein (ORF VI), partial CSB-YP312263CFH
CSB-EP312263CFH
CSB-BP312263CFH
CSB-MP312263CFH
CSB-EP312263CFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Serine/threonine-protein phosphatase 2B catalytic subunit (cna-1), partial CSB-YP312264NHA
CSB-EP312264NHA
CSB-BP312264NHA
CSB-MP312264NHA
CSB-EP312264NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Carbon storage regulator homolog (csrA) CSB-YP312265LET
CSB-EP312265LET
CSB-BP312265LET
CSB-MP312265LET
CSB-EP312265LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP312266LET
CSB-EP312266LET
CSB-BP312266LET
CSB-MP312266LET
CSB-EP312266LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira borgpetersenii serovar Hardjo-bovis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP312267LET
CSB-EP312267LET
CSB-BP312267LET
CSB-MP312267LET
CSB-EP312267LET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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