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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saimiriine herpesvirus 2 Gene 18 protein (18) CSB-YP312431SAX
CSB-EP312431SAX
CSB-BP312431SAX
CSB-MP312431SAX
CSB-EP312431SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Gene 24 protein (24), partial CSB-YP312432SAX
CSB-EP312432SAX
CSB-BP312432SAX
CSB-MP312432SAX
CSB-EP312432SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Putative transcription activator BRLF1 homolog (50), partial CSB-YP312433SAX
CSB-EP312433SAX
CSB-BP312433SAX
CSB-MP312433SAX
CSB-EP312433SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Uncharacterized gene 41 protein (41) CSB-YP312434SAX
CSB-EP312434SAX
CSB-BP312434SAX
CSB-MP312434SAX
CSB-EP312434SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 G-protein coupled receptor homolog ECRF3 (74), partial CSB-YP312435SAX1
CSB-EP312435SAX1
CSB-BP312435SAX1
CSB-MP312435SAX1
CSB-EP312435SAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Gene 55 protein (55) CSB-YP312436SAX
CSB-EP312436SAX
CSB-BP312436SAX
CSB-MP312436SAX
CSB-EP312436SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Capsid assembly protein 63 (63), partial CSB-YP312437SAX
CSB-EP312437SAX
CSB-BP312437SAX
CSB-MP312437SAX
CSB-EP312437SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Enhancer of split mbeta protein (HLHmbeta) CSB-YP312438DLU
CSB-EP312438DLU
CSB-BP312438DLU
CSB-MP312438DLU
CSB-EP312438DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P22 Protein gp14 (14) CSB-YP312439EDG
CSB-EP312439EDG
CSB-BP312439EDG
CSB-MP312439EDG
CSB-EP312439EDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cauliflower mosaic virus Aphid transmission protein (ORF II) CSB-YP312440CFH
CSB-EP312440CFH
CSB-BP312440CFH
CSB-MP312440CFH
CSB-EP312440CFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipomyces kononenkoae Alpha-amylase 1 (LKA1), partial CSB-YP312441LGO
CSB-EP312441LGO
CSB-BP312441LGO
CSB-MP312441LGO
CSB-EP312441LGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectin lyase A (pelA) CSB-YP312442DOZ
CSB-EP312442DOZ
CSB-BP312442DOZ
CSB-MP312442DOZ
CSB-EP312442DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides RNA polymerase sigma-54 factor (rpoN) CSB-YP312443RLE
CSB-EP312443RLE
CSB-BP312443RLE
CSB-MP312443RLE
CSB-EP312443RLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mucor mucedo 4-dihydromethyl-trisporate dehydrogenase (tdh) CSB-YP312444MIX
CSB-EP312444MIX
CSB-BP312444MIX
CSB-MP312444MIX
CSB-EP312444MIX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein B17 (VACWR198) CSB-YP312445VAI
CSB-EP312445VAI
CSB-BP312445VAI
CSB-MP312445VAI
CSB-EP312445VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein B5 (PS/HR), partial CSB-YP312446VAI 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Yop proteins translocation protein C (yscC), partial CSB-YP312447YAQ
CSB-EP312447YAQ
CSB-BP312447YAQ
CSB-MP312447YAQ
CSB-EP312447YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yop proteins translocation protein H (yscH) CSB-YP312448YAQ
CSB-EP312448YAQ
CSB-BP312448YAQ
CSB-MP312448YAQ
CSB-EP312448YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica Yop proteins translocation protein M (yscM) CSB-YP312449YAQ
CSB-BP312449YAQ
CSB-MP312449YAQ
CSB-EP312449YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. Hydantoin utilization protein B (hyuB), partial CSB-YP312450PVR
CSB-EP312450PVR
CSB-BP312450PVR
CSB-MP312450PVR
CSB-EP312450PVR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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