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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neurospora crassa Probable E3 ubiquitin ligase complex SCF subunit scon-2 (scon-2), partial CSB-YP312451NHA
CSB-EP312451NHA
CSB-BP312451NHA
CSB-MP312451NHA
CSB-EP312451NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa 40S ribosomal protein S0 (rps-0) CSB-YP312452NHA
CSB-EP312452NHA
CSB-BP312452NHA
CSB-MP312452NHA
CSB-EP312452NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Clock-controlled protein 6 (ccg-6) CSB-YP312453NHA
CSB-EP312453NHA
CSB-BP312453NHA
CSB-MP312453NHA
CSB-EP312453NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized family 31 glucosidase ORF2, partial CSB-YP312454EOS
CSB-EP312454EOS
CSB-BP312454EOS
CSB-MP312454EOS
CSB-EP312454EOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6A Protein U8 (U8) CSB-YP312455HJZ
CSB-EP312455HJZ
CSB-BP312455HJZ
CSB-MP312455HJZ
CSB-EP312455HJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6A Protein U7 (U7) CSB-YP312456HJZ
CSB-EP312456HJZ
CSB-BP312456HJZ
CSB-MP312456HJZ
CSB-EP312456HJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protein bli-3 (bli-3) CSB-YP312457NHA
CSB-EP312457NHA
CSB-BP312457NHA
CSB-MP312457NHA
CSB-EP312457NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Stage III sporulation protein AA (spoIIIAA) CSB-YP312458BRJ
CSB-EP312458BRJ
CSB-BP312458BRJ
CSB-MP312458BRJ
CSB-EP312458BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haliotis cracherodii Egg-lysin CSB-YP312459HAX
CSB-EP312459HAX
CSB-BP312459HAX
CSB-MP312459HAX
CSB-EP312459HAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Moraxella sp. Haloacetate dehalogenase H-2 (dehH2) CSB-YP312460MIE
CSB-EP312460MIE
CSB-BP312460MIE
CSB-MP312460MIE
CSB-EP312460MIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ascaris suum Ovarian abundant message protein (OAM), partial CSB-YP312461DOS
CSB-EP312461DOS
CSB-BP312461DOS
CSB-MP312461DOS
CSB-EP312461DOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Septum site-determining protein MinC (minC) CSB-YP312462BRJ
CSB-EP312462BRJ
CSB-BP312462BRJ
CSB-MP312462BRJ
CSB-EP312462BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenmedipham hydrolase (pcd) CSB-YP312463AOK
CSB-EP312463AOK
CSB-BP312463AOK
CSB-MP312463AOK
CSB-EP312463AOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine viral diarrhea virus Genome polyprotein, partial CSB-YP312464BKX1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Copper-containing nitrite reductase (nirU) CSB-YP312465RCS
CSB-EP312465RCS
CSB-BP312465RCS
CSB-MP312465RCS
CSB-EP312465RCS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dickeya dadantii Lipoprotein outS (outS) CSB-YP312466DKD
CSB-EP312466DKD
CSB-BP312466DKD
CSB-MP312466DKD
CSB-EP312466DKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Cortical cell-delineating protein CSB-YP312467ZAX
CSB-EP312467ZAX
CSB-BP312467ZAX
CSB-MP312467ZAX
CSB-EP312467ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Presenilin spe-4 (spe-4), partial CSB-YP312468CXY
CSB-EP312468CXY
CSB-BP312468CXY
CSB-MP312468CXY
CSB-EP312468CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein jag (jag) CSB-YP312469BRJ
CSB-EP312469BRJ
CSB-BP312469BRJ
CSB-MP312469BRJ
CSB-EP312469BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein unc-33 (unc-33), partial CSB-YP312470CXY
CSB-EP312470CXY
CSB-BP312470CXY
CSB-MP312470CXY
CSB-EP312470CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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