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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ustilago maydis Probable chloride channel protein UM03490-D (UM11084.2), partial CSB-YP313389UBS
CSB-EP313389UBS
CSB-BP313389UBS
CSB-MP313389UBS
CSB-EP313389UBS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectate lyase A (pelA) CSB-YP313390EGI
CSB-EP313390EGI
CSB-BP313390EGI
CSB-MP313390EGI
CSB-EP313390EGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectinesterase A (pemA) CSB-YP313391EGI
CSB-EP313391EGI
CSB-BP313391EGI
CSB-MP313391EGI
CSB-EP313391EGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Chromosome transmission fidelity protein 18 (ctf18), partial CSB-YP313392EKF
CSB-EP313392EKF
CSB-BP313392EKF
CSB-MP313392EKF
CSB-EP313392EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Gamma-glutamyl phosphate reductase (proA) CSB-YP313393DWY
CSB-EP313393DWY
CSB-BP313393DWY
CSB-MP313393DWY
CSB-EP313393DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrroline-5-carboxylate reductase (proC) CSB-YP313394CQK
CSB-EP313394CQK
CSB-BP313394CQK
CSB-MP313394CQK
CSB-EP313394CQK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthopleura elegantissima Toxin APE 1-1 CSB-YP313395DNA
CSB-EP313395DNA
CSB-BP313395DNA
CSB-MP313395DNA
CSB-EP313395DNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bunodosoma granulifera Neurotoxin Bg-3 CSB-YP313396BNL
CSB-EP313396BNL
CSB-BP313396BNL
CSB-MP313396BNL
CSB-EP313396BNL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Histone H2B 7 (H2B-VII) CSB-YP313397CH
CSB-EP313397CH
CSB-BP313397CH
CSB-MP313397CH
CSB-EP313397CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase pin1 (pin1) CSB-YP313398RDE
CSB-EP313398RDE
CSB-BP313398RDE
CSB-MP313398RDE
CSB-EP313398RDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype K Virion infectivity factor (vif) CSB-YP313399HLP
CSB-EP313399HLP
CSB-BP313399HLP
CSB-MP313399HLP
CSB-EP313399HLP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype K Protein Rev (rev) CSB-YP313400HLQ
CSB-EP313400HLQ
CSB-BP313400HLQ
CSB-MP313400HLQ
CSB-EP313400HLQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vespa magnifica Vespid chemotactic peptide 5g CSB-YP313401VBQ
CSB-EP313401VBQ
CSB-BP313401VBQ
CSB-MP313401VBQ
CSB-EP313401VBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus marmoreus Conotoxin Mr3.4 CSB-YP313402DWD
CSB-EP313402DWD
CSB-BP313402DWD
CSB-MP313402DWD
CSB-EP313402DWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) CSB-YP313403FKZ
CSB-EP313403FKZ
CSB-BP313403FKZ
CSB-MP313403FKZ
CSB-EP313403FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vespa analis Mastoparan-A CSB-YP313404VBN
CSB-EP313404VBN
CSB-BP313404VBN
CSB-MP313404VBN
CSB-EP313404VBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hadronyche sp. Delta-hexatoxin-Hsp201a CSB-YP313405HAC
CSB-EP313405HAC
CSB-BP313405HAC
CSB-MP313405HAC
CSB-EP313405HAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transcription antitermination protein NusG (nusG) CSB-YP313406FLG
CSB-EP313406FLG
CSB-BP313406FLG
CSB-MP313406FLG
CSB-EP313406FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus consors Mu-conotoxin CnIIIB CSB-YP313407COF
CSB-EP313407COF
CSB-BP313407COF
CSB-MP313407COF
CSB-EP313407COF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional regulator sarA (sarA) CSB-YP313408FKZ
CSB-EP313408FKZ
CSB-BP313408FKZ
CSB-MP313408FKZ
CSB-EP313408FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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