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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase cyp11 (cyp11) CSB-YP314652RDE
CSB-EP314652RDE
CSB-BP314652RDE
CSB-MP314652RDE
CSB-EP314652RDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizopus oryzae FK506-binding protein 2A (FKBP2), partial CSB-YP314653RDE1
CSB-EP314653RDE1
CSB-BP314653RDE1
CSB-MP314653RDE1
CSB-EP314653RDE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype K Protein Tat CSB-YP314654HLQ
CSB-EP314654HLQ
CSB-BP314654HLQ
CSB-MP314654HLQ
CSB-EP314654HLQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group O Protein Rev (rev) CSB-YP314655HLT
CSB-EP314655HLT
CSB-BP314655HLT
CSB-MP314655HLT
CSB-EP314655HLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype F2 Virion infectivity factor (vif) CSB-YP314656HLO
CSB-EP314656HLO
CSB-BP314656HLO
CSB-MP314656HLO
CSB-EP314656HLO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polistes dominula Dominulin-A CSB-YP314657POG
CSB-EP314657POG
CSB-BP314657POG
CSB-MP314657POG
CSB-EP314657POG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus marmoreus Conotoxin mr3a CSB-YP314658DWD
CSB-EP314658DWD
CSB-BP314658DWD
CSB-MP314658DWD
CSB-EP314658DWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype K Protein Vpr (vpr) CSB-YP314659HLQ
CSB-EP314659HLQ
CSB-BP314659HLQ
CSB-MP314659HLQ
CSB-EP314659HLQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protonectarina sylveirae Protonectin CSB-YP314661PSS
CSB-EP314661PSS
CSB-BP314661PSS
CSB-MP314661PSS
CSB-EP314661PSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA mismatch repair protein MutS (mutS), partial CSB-YP314662FKZ
CSB-EP314662FKZ
CSB-BP314662FKZ
CSB-MP314662FKZ
CSB-EP314662FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA topoisomerase 4 subunit B (parE), partial CSB-YP314663FKZ
CSB-EP314663FKZ
CSB-BP314663FKZ
CSB-MP314663FKZ
CSB-EP314663FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Elicitor peptide 7 (PEP7) CSB-YP314664DOA
CSB-EP314664DOA
CSB-BP314664DOA
CSB-MP314664DOA
CSB-EP314664DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N-acetylmuramoyl-L-alanine amidase sle1 (sle1) CSB-YP314665FKZ
CSB-EP314665FKZ
CSB-BP314665FKZ
CSB-MP314665FKZ
CSB-EP314665FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delta-hemolysin (hld) CSB-YP314666FKZ
CSB-EP314666FKZ
CSB-BP314666FKZ
CSB-MP314666FKZ
CSB-EP314666FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus magus Alpha-conotoxin-like MIA CSB-YP314667COS
CSB-EP314667COS
CSB-BP314667COS
CSB-MP314667COS
CSB-EP314667COS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus obscurus Alpha-conotoxin OIVB CSB-YP314668COX
CSB-EP314668COX
CSB-BP314668COX
CSB-MP314668COX
CSB-EP314668COX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dendroaspis angusticeps Fasciculin-1 CSB-YP314669DBG
CSB-EP314669DBG
CSB-BP314669DBG
CSB-MP314669DBG
CSB-EP314669DBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human T-cell leukemia virus 1 Protein Rex CSB-YP314670HQG
CSB-EP314670HQG
CSB-BP314670HQG
CSB-MP314670HQG
CSB-EP314670HQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human T-cell leukemia virus 1 Gag-Pro-Pol polyprotein (gag-pro-pol), partial CSB-YP314671HQH
CSB-EP314671HQH
CSB-BP314671HQH
CSB-MP314671HQH
CSB-EP314671HQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium perfringens Phospholipase C (plc) CSB-YP314672CMB
CSB-BP314672CMB
CSB-MP314672CMB
CSB-EP314672CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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