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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Breda virus 1 Hemagglutinin-esterase (HE), partial CSB-YP314632BWJ1
CSB-EP314632BWJ1
CSB-BP314632BWJ1
CSB-MP314632BWJ1
CSB-EP314632BWJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fragaria ananassa Profilin CSB-YP314633FEZ
CSB-EP314633FEZ
CSB-BP314633FEZ
CSB-MP314633FEZ
CSB-EP314633FEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Reaction center protein L chain (pufL), partial CSB-YP314634RLE1
CSB-EP314634RLE1
CSB-BP314634RLE1
CSB-MP314634RLE1
CSB-EP314634RLE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Elongation factor P (efp) CSB-YP314635BWS
CSB-EP314635BWS
CSB-BP314635BWS
CSB-MP314635BWS
CSB-EP314635BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Translation initiation factor IF-1 (infA) CSB-YP314636BWS
CSB-EP314636BWS
CSB-BP314636BWS
CSB-MP314636BWS
CSB-EP314636BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Isoleucine--tRNA ligase (ileS), partial CSB-YP314637BWS
CSB-EP314637BWS
CSB-BP314637BWS
CSB-MP314637BWS
CSB-EP314637BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative auxin-responsive protein IAA29 (IAA29) CSB-YP314638OFG
CSB-EP314638OFG
CSB-BP314638OFG
CSB-MP314638OFG
CSB-EP314638OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus Nuclear export protein (NS) CSB-YP314639IKI
CSB-EP314639IKI
CSB-BP314639IKI
CSB-MP314639IKI
CSB-EP314639IKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Urease accessory protein UreE (ureE) CSB-YP314640BWS
CSB-EP314640BWS
CSB-BP314640BWS
CSB-MP314640BWS
CSB-EP314640BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides elegans Potassium channel toxin alpha-KTx 2.12 CSB-YP314641CFW
CSB-EP314641CFW
CSB-BP314641CFW
CSB-MP314641CFW
CSB-EP314641CFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides noxius Potassium channel toxin alpha-KTx 1.11 CSB-YP314642DRB
CSB-EP314642DRB
CSB-BP314642DRB
CSB-MP314642DRB
CSB-EP314642DRB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NAD (P) transhydrogenase subunit beta, partial CSB-YP314643RMA1
CSB-EP314643RMA1
CSB-BP314643RMA1
CSB-MP314643RMA1
CSB-EP314643RMA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans FK506-binding protein 4 (fpr4) CSB-YP314644EKF
CSB-EP314644EKF
CSB-BP314644EKF
CSB-MP314644EKF
CSB-EP314644EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parabuthus transvaalicus Bestoxin CSB-YP314645PBV
CSB-EP314645PBV
CSB-BP314645PBV
CSB-MP314645PBV
CSB-EP314645PBV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 4 Protein TusB (tusB) CSB-YP314646SGE
CSB-EP314646SGE
CSB-BP314646SGE
CSB-MP314646SGE
CSB-EP314646SGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Pyrroline-5-carboxylate reductase (proC) CSB-YP314647DWY
CSB-EP314647DWY
CSB-BP314647DWY
CSB-MP314647DWY
CSB-EP314647DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Uncharacterized protein Cgl2355/cg2587 (Cgl2355, cg2587) CSB-YP314648DWY
CSB-EP314648DWY
CSB-BP314648DWY
CSB-MP314648DWY
CSB-EP314648DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heme exporter protein D (ccmD), partial CSB-YP314649FFT
CSB-EP314649FFT
CSB-BP314649FFT
CSB-MP314649FFT
CSB-EP314649FFT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Histone H2B 5 (H2B-V) CSB-YP314650CH
CSB-EP314650CH
CSB-BP314650CH
CSB-MP314650CH
CSB-EP314650CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase A1 (cyp2) CSB-YP314651RDE
CSB-EP314651RDE
CSB-BP314651RDE
CSB-MP314651RDE
CSB-EP314651RDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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