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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP316273SMV
CSB-EP316273SMV
CSB-BP316273SMV
CSB-MP316273SMV
CSB-EP316273SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP316274SMV
CSB-EP316274SMV
CSB-BP316274SMV
CSB-MP316274SMV
CSB-EP316274SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP316275SMW
CSB-EP316275SMW
CSB-BP316275SMW
CSB-MP316275SMW
CSB-EP316275SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine regulator (argR1) CSB-YP316276SMV
CSB-EP316276SMV
CSB-BP316276SMV
CSB-MP316276SMV
CSB-EP316276SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-dehydroquinate dehydratase (aroD) CSB-YP316277SMV
CSB-EP316277SMV
CSB-BP316277SMV
CSB-MP316277SMV
CSB-EP316277SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-dehydroquinate synthase (aroB) CSB-YP316278SMV
CSB-EP316278SMV
CSB-BP316278SMV
CSB-MP316278SMV
CSB-EP316278SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein spx (spxA) CSB-YP316279SMV
CSB-EP316279SMV
CSB-BP316279SMV
CSB-MP316279SMV
CSB-EP316279SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Glutamate racemase (murI) CSB-YP316280SMW
CSB-EP316280SMW
CSB-BP316280SMW
CSB-MP316280SMW
CSB-EP316280SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Uncharacterized membrane protein SPs1599 (SPs1599), partial CSB-YP316281SMW1
CSB-EP316281SMW1
CSB-BP316281SMW1
CSB-MP316281SMW1
CSB-EP316281SMW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 CCA-adding enzyme (cca) CSB-YP316282SMW
CSB-EP316282SMW
CSB-BP316282SMW
CSB-MP316282SMW
CSB-EP316282SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 60 kDa chaperonin (groL), partial CSB-YP316283SMW
CSB-EP316283SMW
CSB-BP316283SMW
CSB-MP316283SMW
CSB-EP316283SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 10 kDa chaperonin (groS) CSB-YP316284SMW
CSB-EP316284SMW
CSB-BP316284SMW
CSB-MP316284SMW
CSB-EP316284SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Probable apo-citrate lyase phosphoribosyl-dephospho-CoA transferase (citX) CSB-YP316286SMW
CSB-EP316286SMW
CSB-BP316286SMW
CSB-MP316286SMW
CSB-EP316286SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytidylate kinase (cmk) CSB-YP316287SMV
CSB-EP316287SMV
CSB-BP316287SMV
CSB-MP316287SMV
CSB-EP316287SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphopantetheine adenylyltransferase (coaD) CSB-YP316288SMV
CSB-EP316288SMV
CSB-BP316288SMV
CSB-MP316288SMV
CSB-EP316288SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP316289SMV
CSB-EP316289SMV
CSB-BP316289SMV
CSB-MP316289SMV
CSB-EP316289SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Major cold shock protein (cspA) CSB-YP316290SMW
CSB-EP316290SMW
CSB-BP316290SMW
CSB-MP316290SMW
CSB-EP316290SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DegV domain-containing protein SpyM3_1149 (SpyM3_1149) CSB-YP316291SMV
CSB-EP316291SMV
CSB-BP316291SMV
CSB-MP316291SMV
CSB-EP316291SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deoxyribose-phosphate aldolase (deoC) CSB-YP316292SMV
CSB-EP316292SMV
CSB-BP316292SMV
CSB-MP316292SMV
CSB-EP316292SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-alanine--poly (phosphoribitol) ligase subunit 1, partial CSB-YP316293SMV
CSB-EP316293SMV
CSB-BP316293SMV
CSB-MP316293SMV
CSB-EP316293SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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