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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP316294SMV
CSB-EP316294SMV
CSB-BP316294SMV
CSB-MP316294SMV
CSB-EP316294SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Chaperone protein DnaJ (dnaJ) CSB-YP316295SMW
CSB-EP316295SMW
CSB-BP316295SMW
CSB-MP316295SMW
CSB-EP316295SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 DNA polymerase IV (dinB) CSB-YP316296SMW
CSB-EP316296SMW
CSB-BP316296SMW
CSB-MP316296SMW
CSB-EP316296SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Elongation factor P (efp) CSB-YP316297SMW
CSB-EP316297SMW
CSB-BP316297SMW
CSB-MP316297SMW
CSB-EP316297SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enolase (eno) CSB-YP316298SMV
CSB-EP316298SMV
CSB-BP316298SMV
CSB-MP316298SMV
CSB-EP316298SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP316299SMV
CSB-EP316299SMV
CSB-BP316299SMV
CSB-MP316299SMV
CSB-EP316299SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (folK) CSB-YP316300SMW
CSB-EP316300SMW
CSB-BP316300SMW
CSB-MP316300SMW
CSB-EP316300SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha-glycerophosphate oxidase (glpO), partial CSB-YP316301SMV
CSB-EP316301SMV
CSB-BP316301SMV
CSB-MP316301SMV
CSB-EP316301SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP316302SMW
CSB-EP316302SMW
CSB-BP316302SMW
CSB-MP316302SMW
CSB-EP316302SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP316303SMV
CSB-EP316303SMV
CSB-BP316303SMV
CSB-MP316303SMV
CSB-EP316303SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Phosphoglucosamine mutase (glmM) CSB-YP316304SMW
CSB-EP316304SMW
CSB-BP316304SMW
CSB-MP316304SMW
CSB-EP316304SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP316305SMV
CSB-EP316305SMV
CSB-BP316305SMV
CSB-MP316305SMV
CSB-EP316305SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTPase obg (obg) CSB-YP316306SMV
CSB-EP316306SMV
CSB-BP316306SMV
CSB-MP316306SMV
CSB-EP316306SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable potassium transport system protein kup (kup), partial CSB-YP316307SMV
CSB-EP316307SMV
CSB-BP316307SMV
CSB-MP316307SMV
CSB-EP316307SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Non-canonical purine NTP pyrophosphatase (SPs1596) CSB-YP316308SMW
CSB-EP316308SMW
CSB-BP316308SMW
CSB-MP316308SMW
CSB-EP316308SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-binding protein HU (hup) CSB-YP316309SMV
CSB-EP316309SMV
CSB-BP316309SMV
CSB-MP316309SMV
CSB-EP316309SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 33 kDa chaperonin (hslO) CSB-YP316310SMV
CSB-EP316310SMV
CSB-BP316310SMV
CSB-MP316310SMV
CSB-EP316310SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Redox-sensing transcriptional repressor rex (rex) CSB-YP316311SMW
CSB-EP316311SMW
CSB-BP316311SMW
CSB-MP316311SMW
CSB-EP316311SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazolonepropionase (hutI) CSB-YP316312SMV
CSB-EP316312SMV
CSB-BP316312SMV
CSB-MP316312SMV
CSB-EP316312SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GMP reductase (guaC) CSB-YP316313SMV
CSB-EP316313SMV
CSB-BP316313SMV
CSB-MP316313SMV
CSB-EP316313SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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