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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Conus distans Conotoxin di16a CSB-YP316334COH
CSB-EP316334COH
CSB-BP316334COH
CSB-MP316334COH
CSB-EP316334COH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus stigmurus Pape peptide CSB-YP316335TEV
CSB-EP316335TEV
CSB-BP316335TEV
CSB-MP316335TEV
CSB-EP316335TEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus stigmurus Peptide 2900 CSB-YP316336TEV
CSB-EP316336TEV
CSB-BP316336TEV
CSB-MP316336TEV
CSB-EP316336TEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Outer membrane protein A (ompA), partial CSB-YP316337EGZ1
CSB-EP316337EGZ1
CSB-BP316337EGZ1
CSB-MP316337EGZ1
CSB-EP316337EGZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fimbrillin matB homolog (matB) CSB-YP316338ENL
CSB-EP316338ENL
CSB-BP316338ENL
CSB-MP316338ENL
CSB-EP316338ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus leopardus Conotoxin Leo-T1 CSB-YP316339CXD
CSB-EP316339CXD
CSB-BP316339CXD
CSB-MP316339CXD
CSB-EP316339CXD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amycolatopsis sp. Amythiamicin A/B CSB-YP316340AJG
CSB-EP316340AJG
CSB-BP316340AJG
CSB-MP316340AJG
CSB-EP316340AJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops insularis Bothroinsularin subunit alpha CSB-YP316341BIX
CSB-EP316341BIX
CSB-BP316341BIX
CSB-MP316341BIX
CSB-EP316341BIX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis NAD-specific glutamate dehydrogenase (gdh) CSB-YP316342EYA
CSB-EP316342EYA
CSB-BP316342EYA
CSB-MP316342EYA
CSB-EP316342EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja atra Cytotoxin najalgesin CSB-YP316343NFG
CSB-EP316343NFG
CSB-BP316343NFG
CSB-MP316343NFG
CSB-EP316343NFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Small COPII coat GTPase SAR1 (sar1) CSB-YP316344AVE
CSB-EP316344AVE
CSB-BP316344AVE
CSB-MP316344AVE
CSB-EP316344AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Lysophospholipase 1 (plb1), partial CSB-YP316345NGS
CSB-EP316345NGS
CSB-BP316345NGS
CSB-MP316345NGS
CSB-EP316345NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Putative DNA-directed RNA polymerase subunit 10 homolog (Mal-103) CSB-YP316346AYE
CSB-EP316346AYE
CSB-BP316346AYE
CSB-MP316346AYE
CSB-EP316346AYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Probable DNA-directed RNA polymerase subunit 1 homolog (War-109), partial CSB-YP316347AEI
CSB-EP316347AEI
CSB-BP316347AEI
CSB-MP316347AEI
CSB-EP316347AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Putative RNA Helicase B962L (Pret-084), partial CSB-YP316348AYG
CSB-EP316348AYG
CSB-BP316348AYG
CSB-MP316348AYG
CSB-EP316348AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Putative ATP-dependent RNA helicase Q706L (Ken-134), partial CSB-YP316349AEB
CSB-EP316349AEB
CSB-BP316349AEB
CSB-MP316349AEB
CSB-EP316349AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Cysteine protease S273R (War-121) CSB-YP316350AEI
CSB-EP316350AEI
CSB-BP316350AEI
CSB-MP316350AEI
CSB-EP316350AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus NifS-like protein (War-134) CSB-YP316352AEI
CSB-EP316352AEI
CSB-BP316352AEI
CSB-MP316352AEI
CSB-EP316352AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus DNA-directed RNA polymerase subunit 6 homolog (War-079) CSB-YP316353AEI
CSB-EP316353AEI
CSB-BP316353AEI
CSB-MP316353AEI
CSB-EP316353AEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Viral histone-like protein A104R (Pret-047) CSB-YP316354AYG
CSB-EP316354AYG
CSB-BP316354AYG
CSB-MP316354AYG
CSB-EP316354AYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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