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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhodobacter capsulatus Beta-lactamase CSB-YP319656RLC
CSB-EP319656RLC
CSB-BP319656RLC
CSB-MP319656RLC
CSB-EP319656RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Chitin synthase 2 (CHS2), partial CSB-YP319657SVG
CSB-EP319657SVG
CSB-BP319657SVG
CSB-MP319657SVG
CSB-EP319657SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Exopolysaccharide production repressor protein (exoX), partial CSB-YP319658RKX1
CSB-EP319658RKX1
CSB-BP319658RKX1
CSB-MP319658RKX1
CSB-EP319658RKX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli K88 fimbrial protein AD (faeG) CSB-YP319659ENL
CSB-EP319659ENL
CSB-BP319659ENL
CSB-MP319659ENL
CSB-EP319659ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum AAC-rich mRNA clone AAC11 protein (AAC11) CSB-YP319660DKK
CSB-EP319660DKK
CSB-BP319660DKK
CSB-MP319660DKK
CSB-EP319660DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma mansoni Tegument antigen CSB-YP319661SXQ
CSB-EP319661SXQ
CSB-BP319661SXQ
CSB-MP319661SXQ
CSB-EP319661SXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Limulus polyphemus Polyphemusin-1 CSB-YP319662LGC
CSB-EP319662LGC
CSB-BP319662LGC
CSB-MP319662LGC
CSB-EP319662LGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Arylsulfatase (AS), partial CSB-YP319663DSJ
CSB-EP319663DSJ
CSB-BP319663DSJ
CSB-MP319663DSJ
CSB-EP319663DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Fructose-bisphosphate aldolase CSB-YP319664PLO
CSB-EP319664PLO
CSB-BP319664PLO
CSB-MP319664PLO
CSB-EP319664PLO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Photosystem I reaction center subunit V, chloroplastic (PSAG), partial CSB-YP319665DSJ1
CSB-EP319665DSJ1
CSB-BP319665DSJ1
CSB-MP319665DSJ1
CSB-EP319665DSJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type-2 restriction enzyme SmaI (smaIR) CSB-YP319666SYN
CSB-EP319666SYN
CSB-BP319666SYN
CSB-MP319666SYN
CSB-EP319666SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum TGACG-sequence-specific DNA-binding protein TGA-1B (TGA1B) CSB-YP319667NHE
CSB-EP319667NHE
CSB-BP319667NHE
CSB-MP319667NHE
CSB-EP319667NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein K (psbK) CSB-YP319668DZX
CSB-EP319668DZX
CSB-BP319668DZX
CSB-MP319668DZX
CSB-EP319668DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymphocytic choriomeningitis virus RNA-directed RNA polymerase L (L), partial CSB-YP319669LKW
CSB-EP319669LKW
CSB-BP319669LKW
CSB-MP319669LKW
CSB-EP319669LKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Modification methylase Cfr9I (cfr9IM) CSB-YP319670DTO
CSB-EP319670DTO
CSB-BP319670DTO
CSB-MP319670DTO
CSB-EP319670DTO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii DNA-directed RNA polymerase subunit A' (rpoA2) CSB-YP319671MNS
CSB-EP319671MNS
CSB-BP319671MNS
CSB-MP319671MNS
CSB-EP319671MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fibrobacter succinogenes Endoglucanase 3 (cel-3) CSB-YP319672FER
CSB-EP319672FER
CSB-BP319672FER
CSB-MP319672FER
CSB-EP319672FER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sendai virus Phosphoprotein (P/V/C), partial CSB-YP319673SEX
CSB-EP319673SEX
CSB-BP319673SEX
CSB-MP319673SEX
CSB-EP319673SEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine adenovirus serotype 2 Hexon-associated protein (PIX) CSB-YP319674CCN
CSB-EP319674CCN
CSB-BP319674CCN
CSB-MP319674CCN
CSB-EP319674CCN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Chlorophyll a-b binding protein 3B, chloroplastic (CAB3B), partial CSB-YP319675SZY1
CSB-EP319675SZY1
CSB-BP319675SZY1
CSB-MP319675SZY1
CSB-EP319675SZY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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