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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Phosphate import ATP-binding protein PstB 1 (pstB1) CSB-YP317874SMV
CSB-EP317874SMV
CSB-BP317874SMV
CSB-MP317874SMV
CSB-EP317874SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate import ATP-binding protein PstB 2 (pstB2) CSB-YP317875SMV
CSB-EP317875SMV
CSB-BP317875SMV
CSB-MP317875SMV
CSB-EP317875SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ABC transporter ATP-binding protein SpyM3_0208 (SpyM3_0208) CSB-YP317876SMV
CSB-EP317876SMV
CSB-BP317876SMV
CSB-MP317876SMV
CSB-EP317876SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Acetate kinase (ackA) CSB-YP317877SMW
CSB-EP317877SMW
CSB-BP317877SMW
CSB-MP317877SMW
CSB-EP317877SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 ATP-dependent helicase/deoxyribonuclease subunit B (rexB), partial CSB-YP317878SMW
CSB-EP317878SMW
CSB-BP317878SMW
CSB-MP317878SMW
CSB-EP317878SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fructose-bisphosphate aldolase (fba) CSB-YP317879SMV
CSB-EP317879SMV
CSB-BP317879SMV
CSB-MP317879SMV
CSB-EP317879SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Formimidoylglutamase (hutG) CSB-YP317880SMW
CSB-EP317880SMW
CSB-BP317880SMW
CSB-MP317880SMW
CSB-EP317880SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-phosphoshikimate 1-carboxyvinyltransferase (aroA.1) CSB-YP317881SMV
CSB-EP317881SMV
CSB-BP317881SMV
CSB-MP317881SMV
CSB-EP317881SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 3-dehydroquinate synthase (aroB) CSB-YP317882SMW
CSB-EP317882SMW
CSB-BP317882SMW
CSB-MP317882SMW
CSB-EP317882SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Stress response regulator gls24 homolog (SPs1097) CSB-YP317883SMW
CSB-EP317883SMW
CSB-BP317883SMW
CSB-MP317883SMW
CSB-EP317883SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase epsilon chain (atpC) CSB-YP317884SMV
CSB-EP317884SMV
CSB-BP317884SMV
CSB-MP317884SMV
CSB-EP317884SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 V-type ATP synthase alpha chain (atpA), partial CSB-YP317885SMW
CSB-EP317885SMW
CSB-BP317885SMW
CSB-MP317885SMW
CSB-EP317885SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized membrane protein SpyM3_0260 (SpyM3_0260), partial CSB-YP317886SMV1
CSB-EP317886SMV1
CSB-BP317886SMV1
CSB-MP317886SMV1
CSB-EP317886SMV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP317887SMV
CSB-EP317887SMV
CSB-BP317887SMV
CSB-MP317887SMV
CSB-EP317887SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol kinase (glpK), partial CSB-YP317888SMV
CSB-EP317888SMV
CSB-BP317888SMV
CSB-MP317888SMV
CSB-EP317888SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP317889SMV
CSB-EP317889SMV
CSB-BP317889SMV
CSB-MP317889SMV
CSB-EP317889SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative competence-damage inducible protein (cinA) CSB-YP317890SMV
CSB-EP317890SMV
CSB-BP317890SMV
CSB-MP317890SMV
CSB-EP317890SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Citrate lyase acyl carrier protein (citD) CSB-YP317891SMW
CSB-EP317891SMW
CSB-BP317891SMW
CSB-MP317891SMW
CSB-EP317891SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP317892SMV
CSB-EP317892SMV
CSB-BP317892SMV
CSB-MP317892SMV
CSB-EP317892SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Phosphopantetheine adenylyltransferase (coaD) CSB-YP317893SMW
CSB-EP317893SMW
CSB-BP317893SMW
CSB-MP317893SMW
CSB-EP317893SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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