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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Protein rolA (rolA) CSB-YP318901AEW
CSB-EP318901AEW
CSB-BP318901AEW
CSB-MP318901AEW
CSB-EP318901AEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pleurodeles waltl Hemoglobin larval subunit alpha CSB-YP318902EWZ
CSB-EP318902EWZ
CSB-BP318902EWZ
CSB-MP318902EWZ
CSB-EP318902EWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colicin-D immunity protein (cdi) CSB-YP318903ENL
CSB-EP318903ENL
CSB-BP318903ENL
CSB-MP318903ENL
CSB-EP318903ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmid segregation protein parM (parM) CSB-YP318904ENL
CSB-EP318904ENL
CSB-BP318904ENL
CSB-MP318904ENL
CSB-EP318904ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 11.1 kDa protein CSB-YP318905ENL
CSB-EP318905ENL
CSB-BP318905ENL
CSB-MP318905ENL
CSB-EP318905ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lumbricus terrestris Lombricine kinase CSB-YP318906LJN
CSB-EP318906LJN
CSB-BP318906LJN
CSB-MP318906LJN
CSB-EP318906LJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aplysia californica Califin-C CSB-YP318907DNM
CSB-EP318907DNM
CSB-BP318907DNM
CSB-MP318907DNM
CSB-EP318907DNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Blastoderm-specific protein 25D (Bsg25D), partial CSB-YP318908DLU
CSB-EP318908DLU
CSB-BP318908DLU
CSB-MP318908DLU
CSB-EP318908DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare 26 kDa endochitinase 1 CSB-YP318909HWQ
CSB-EP318909HWQ
CSB-BP318909HWQ
CSB-MP318909HWQ
CSB-EP318909HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Superoxide dismutase [Cu-Zn], chloroplastic (SODCP) CSB-YP318910EWE
CSB-EP318910EWE
CSB-BP318910EWE
CSB-MP318910EWE
CSB-EP318910EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus nigra Plastocyanin B, chloroplastic (PETE) CSB-YP318911PPT
CSB-EP318911PPT
CSB-BP318911PPT
CSB-MP318911PPT
CSB-EP318911PPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Prestalk protein (ecmB), partial CSB-YP318912DKK
CSB-EP318912DKK
CSB-BP318912DKK
CSB-MP318912DKK
CSB-EP318912DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Inositol-3-phosphate synthase (INO1), partial CSB-YP318913SVG
CSB-EP318913SVG
CSB-BP318913SVG
CSB-MP318913SVG
CSB-EP318913SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cryptic beta-glucoside bgl operon antiterminator (bglG) CSB-YP318914ENV
CSB-EP318914ENV
CSB-BP318914ENV
CSB-MP318914ENV
CSB-EP318914ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Gluconokinase (gntK), partial CSB-YP318915BRJ
CSB-EP318915BRJ
CSB-BP318915BRJ
CSB-MP318915BRJ
CSB-EP318915BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Apomucin, partial CSB-YP318916PI
CSB-EP318916PI
CSB-BP318916PI
CSB-MP318916PI
CSB-EP318916PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus oreganus concolor Myotoxin-1 CSB-YP318917DYF
CSB-EP318917DYF
CSB-BP318917DYF
CSB-MP318917DYF
CSB-EP318917DYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP318918BRJ
CSB-EP318918BRJ
CSB-BP318918BRJ
CSB-MP318918BRJ
CSB-EP318918BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis N5-carboxyaminoimidazole ribonucleotide mutase (purE) CSB-YP318919BRJ
CSB-EP318919BRJ
CSB-BP318919BRJ
CSB-MP318919BRJ
CSB-EP318919BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0062 protein yexA (yexA) CSB-YP318920BRJ
CSB-EP318920BRJ
CSB-BP318920BRJ
CSB-MP318920BRJ
CSB-EP318920BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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