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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhizobium meliloti Sulfate adenylyltransferase subunit 2 (nodP) CSB-YP320226RKU
CSB-EP320226RKU
CSB-BP320226RKU
CSB-MP320226RKU
CSB-EP320226RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Nitrile hydratase subunit alpha (nthA) CSB-YP320227RLJ
CSB-EP320227RLJ
CSB-BP320227RLJ
CSB-MP320227RLJ
CSB-EP320227RLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 3-methylcatechol 2,3-dioxygenase (todE) CSB-YP320228PZN
CSB-EP320228PZN
CSB-BP320228PZN
CSB-MP320228PZN
CSB-EP320228PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Nuclease sbcCD subunit C (sbcC), partial CSB-YP320229ENV
CSB-EP320229ENV
CSB-BP320229ENV
CSB-MP320229ENV
CSB-EP320229ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant T-DNA border endonuclease virD1 (virD1) CSB-YP320230AEW
CSB-EP320230AEW
CSB-BP320230AEW
CSB-MP320230AEW
CSB-EP320230AEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Gelation factor (abpC), partial CSB-YP320231DKK
CSB-EP320231DKK
CSB-BP320231DKK
CSB-MP320231DKK
CSB-EP320231DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster DNA-binding protein modulo (mod), partial CSB-YP320232DLU
CSB-EP320232DLU
CSB-BP320232DLU
CSB-MP320232DLU
CSB-EP320232DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colicin-E6 immunity protein (imm) CSB-YP320233ENL
CSB-EP320233ENL
CSB-BP320233ENL
CSB-MP320233ENL
CSB-EP320233ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Uncharacterized protein RA0666 (RA0666) CSB-YP320234RKU
CSB-EP320234RKU
CSB-BP320234RKU
CSB-MP320234RKU
CSB-EP320234RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hadronyche versuta Delta-hexatoxin-Hv1a CSB-YP320235HAD
CSB-EP320235HAD
CSB-BP320235HAD
CSB-MP320235HAD
CSB-EP320235HAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Cobalt-zinc-cadmium resistance protein CzcC (czcC) CSB-YP320236RJG
CSB-EP320236RJG
CSB-BP320236RJG
CSB-MP320236RJG
CSB-EP320236RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Lipoprotein signal peptidase (lspA), partial CSB-YP320237EKO1
CSB-EP320237EKO1
CSB-BP320237EKO1
CSB-MP320237EKO1
CSB-EP320237EKO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P2 Overcoming lysogenization defect protein (old), partial CSB-YP320238EDE
CSB-EP320238EDE
CSB-BP320238EDE
CSB-MP320238EDE
CSB-EP320238EDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma japonicum Immunogenic miracidial antigen 8I (8I) CSB-YP320239SXP
CSB-EP320239SXP
CSB-BP320239SXP
CSB-MP320239SXP
CSB-EP320239SXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus C-phycocyanin alpha chain (cpcA1) CSB-YP320240FPY
CSB-EP320240FPY
CSB-BP320240FPY
CSB-MP320240FPY
CSB-EP320240FPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Myosin heavy chain, skeletal muscle, adult, partial CSB-YP320241CH
CSB-EP320241CH
CSB-BP320241CH
CSB-MP320241CH
CSB-EP320241CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aequipecten irradians Myosin regulatory light chain, striated adductor muscle CSB-YP320242ADN
CSB-EP320242ADN
CSB-BP320242ADN
CSB-MP320242ADN
CSB-EP320242ADN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Elongation factor 1-alpha, somatic form (eef1as) CSB-YP320243XBE
CSB-EP320243XBE
CSB-BP320243XBE
CSB-MP320243XBE
CSB-EP320243XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Nitrogen regulatory protein P-II (glnB) CSB-YP320244RLC
CSB-EP320244RLC
CSB-BP320244RLC
CSB-MP320244RLC
CSB-EP320244RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Red clover mottle virus RNA2 polyprotein CSB-YP320245RCA
CSB-EP320245RCA
CSB-BP320245RCA
CSB-MP320245RCA
CSB-EP320245RCA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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