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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Petunia hybrida Chalcone--flavonone isomerase B (CHI2) CSB-YP320022EUD
CSB-EP320022EUD
CSB-BP320022EUD
CSB-MP320022EUD
CSB-EP320022EUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yggC (yggC) CSB-YP320023ENV
CSB-EP320023ENV
CSB-BP320023ENV
CSB-MP320023ENV
CSB-EP320023ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogen regulatory protein P-II (glnB) CSB-YP320024KBE
CSB-EP320024KBE
CSB-BP320024KBE
CSB-MP320024KBE
CSB-EP320024KBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera berteriana Uncharacterized 8.5 kDa protein CSB-YP320025OAR
CSB-EP320025OAR
CSB-BP320025OAR
CSB-MP320025OAR
CSB-EP320025OAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calliphora vicina Tyrosine-protein kinase ABL (ABL), partial CSB-YP320026CAX
CSB-EP320026CAX
CSB-BP320026CAX
CSB-MP320026CAX
CSB-EP320026CAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato bushy stunt virus Capsid protein (ORF2) CSB-YP320027TGA
CSB-EP320027TGA
CSB-BP320027TGA
CSB-MP320027TGA
CSB-EP320027TGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa HTH-type transcriptional regulator trpI (trpI) CSB-YP320028EZX
CSB-EP320028EZX
CSB-BP320028EZX
CSB-MP320028EZX
CSB-EP320028EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Medicago sativa Early nodulin-75 (ENOD2) CSB-YP320029MQO
CSB-EP320029MQO
CSB-BP320029MQO
CSB-MP320029MQO
CSB-EP320029MQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Cuticle protein 36 CSB-YP320030LHY
CSB-EP320030LHY
CSB-BP320030LHY
CSB-MP320030LHY
CSB-EP320030LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pheretima sieboldi Extracellular globin-1 CSB-YP320031PHK
CSB-EP320031PHK
CSB-BP320031PHK
CSB-MP320031PHK
CSB-EP320031PHK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Pheromone receptor transcription factor (MCM1) CSB-YP320032SVG
CSB-EP320032SVG
CSB-BP320032SVG
CSB-MP320032SVG
CSB-EP320032SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tadarida brasiliensis Hemoglobin subunit alpha-1 CSB-YP320033TAI
CSB-EP320033TAI
CSB-BP320033TAI
CSB-MP320033TAI
CSB-EP320033TAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fimbrial protein P9-2 (pilE) CSB-YP320034NFZ
CSB-EP320034NFZ
CSB-BP320034NFZ
CSB-MP320034NFZ
CSB-EP320034NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase SCH9 (SCH9), partial CSB-YP320035SVG
CSB-EP320035SVG
CSB-BP320035SVG
CSB-MP320035SVG
CSB-EP320035SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Ornithine carbamoyltransferase, mitochondrial (argB) CSB-YP320036EKF
CSB-EP320036EKF
CSB-BP320036EKF
CSB-MP320036EKF
CSB-EP320036EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus F serotype 40 Hexon-associated protein (PVIII) CSB-YP320037HIR
CSB-EP320037HIR
CSB-BP320037HIR
CSB-MP320037HIR
CSB-EP320037HIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinapis alba Photosystem Q (B) protein, partial CSB-YP320038SZO1
CSB-EP320038SZO1
CSB-BP320038SZO1
CSB-MP320038SZO1
CSB-EP320038SZO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid glycoprotein VP7 CSB-YP320039ROI
CSB-EP320039ROI
CSB-BP320039ROI
CSB-MP320039ROI
CSB-EP320039ROI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yhaB (yhaB) CSB-YP320040ENV
CSB-EP320040ENV
CSB-BP320040ENV
CSB-MP320040ENV
CSB-EP320040ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Suid herpesvirus 1 Major DNA-binding protein (DBP), partial CSB-YP320041SRH
CSB-EP320041SRH
CSB-BP320041SRH
CSB-MP320041SRH
CSB-EP320041SRH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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