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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Heat shock 70 kDa protein (HSP70), partial CSB-YP320434LOG
CSB-EP320434LOG
CSB-BP320434LOG
CSB-MP320434LOG
CSB-EP320434LOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Carbamoyl-phosphate synthase small chain (carA) CSB-YP320435SXB
CSB-EP320435SXB
CSB-BP320435SXB
CSB-MP320435SXB
CSB-EP320435SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coat protein CSB-YP320436PDW
CSB-EP320436PDW
CSB-BP320436PDW
CSB-MP320436PDW
CSB-EP320436PDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Seed linoleate 9S-lipoxygenase-2 (LOX1.2), partial CSB-YP320437EWE
CSB-EP320437EWE
CSB-BP320437EWE
CSB-MP320437EWE
CSB-EP320437EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica napus Acetolactate synthase 2, chloroplastic, partial CSB-YP320438BWD
CSB-EP320438BWD
CSB-BP320438BWD
CSB-MP320438BWD
CSB-EP320438BWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon C-phycocyanin-3 beta chain (cpcB3) CSB-YP320439FCY
CSB-EP320439FCY
CSB-BP320439FCY
CSB-MP320439FCY
CSB-EP320439FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon Phycobilisome 30.8 kDa linker polypeptide, phycocyanin-associated, rod (cpcH3) CSB-YP320440FCY
CSB-EP320440FCY
CSB-BP320440FCY
CSB-MP320440FCY
CSB-EP320440FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Actin-2 CSB-YP320441PMB
CSB-EP320441PMB
CSB-BP320441PMB
CSB-MP320441PMB
CSB-EP320441PMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein nifX (nifX) CSB-YP320442DPX
CSB-EP320442DPX
CSB-BP320442DPX
CSB-MP320442DPX
CSB-EP320442DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase-stabilizing/protective protein nifW (nifW) CSB-YP320443DPX
CSB-EP320443DPX
CSB-BP320443DPX
CSB-MP320443DPX
CSB-EP320443DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 (HMG1), partial CSB-YP320444DOA1
CSB-EP320444DOA1
CSB-BP320444DOA1
CSB-MP320444DOA1
CSB-EP320444DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canavalia gladiata Concanavalin-A CSB-YP320445CCB
CSB-EP320445CCB
CSB-BP320445CCB
CSB-MP320445CCB
CSB-EP320445CCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Low molecular mass early light-inducible protein HV60, chloroplastic, partial CSB-YP320446HWQ1
CSB-EP320446HWQ1
CSB-BP320446HWQ1
CSB-MP320446HWQ1
CSB-EP320446HWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyoglomus thermophilum Alpha-amylase 2 (amyB), partial CSB-YP320447DKI
CSB-EP320447DKI
CSB-BP320447DKI
CSB-MP320447DKI
CSB-EP320447DKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Fruit-specific protein (2A11) CSB-YP320448SZY
CSB-EP320448SZY
CSB-BP320448SZY
CSB-MP320448SZY
CSB-EP320448SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cytochrome B translational activator protein CBS2 (CBS2) CSB-YP320449SVG
CSB-EP320449SVG
CSB-BP320449SVG
CSB-MP320449SVG
CSB-EP320449SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial transcription factor 1 (MTF1) CSB-YP320450SVG
CSB-EP320450SVG
CSB-BP320450SVG
CSB-MP320450SVG
CSB-EP320450SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petroselinum crispum 4-coumarate--CoA ligase 1 (4CL1), partial CSB-YP320451PGL
CSB-EP320451PGL
CSB-BP320451PGL
CSB-MP320451PGL
CSB-EP320451PGL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter marburgensis Uncharacterized protein MTBMA_p00010 (MTBMA_p00010), partial CSB-YP320453MSQ
CSB-EP320453MSQ
CSB-BP320453MSQ
CSB-MP320453MSQ
CSB-EP320453MSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin-like protein in vnf region CSB-YP320454DPX
CSB-EP320454DPX
CSB-BP320454DPX
CSB-MP320454DPX
CSB-EP320454DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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