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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Glycine max Stem 31 kDa glycoprotein (VSPB) CSB-YP318780GGV
CSB-EP318780GGV
CSB-BP318780GGV
CSB-MP318780GGV
CSB-EP318780GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Hemagglutinin (HA), partial CSB-YP318781IIW
CSB-EP318781IIW
CSB-BP318781IIW
CSB-MP318781IIW
CSB-EP318781IIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylate kinase (adk) CSB-YP318782PBZ
CSB-EP318782PBZ
CSB-BP318782PBZ
CSB-MP318782PBZ
CSB-EP318782PBZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phalacrocorax carbo Hemoglobin subunit alpha-A (HBAA) CSB-YP318783PGR
CSB-EP318783PGR
CSB-BP318783PGR
CSB-MP318783PGR
CSB-EP318783PGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 30S ribosomal protein S11 (rpsK) CSB-YP318784GFM
CSB-EP318784GFM
CSB-BP318784GFM
CSB-MP318784GFM
CSB-EP318784GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petunia hybrida Superoxide dismutase [Cu-Zn], chloroplastic (SODCP) CSB-YP318785EUD
CSB-EP318785EUD
CSB-BP318785EUD
CSB-MP318785EUD
CSB-EP318785EUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ribulose bisphosphate carboxylase small chain 1B, chloroplastic (RBCS-1B) CSB-YP318786DOA
CSB-EP318786DOA
CSB-BP318786DOA
CSB-MP318786DOA
CSB-EP318786DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Guanine nucleotide-binding protein alpha-2 subunit (GPA2) CSB-YP318787SVG
CSB-EP318787SVG
CSB-BP318787SVG
CSB-MP318787SVG
CSB-EP318787SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paramecium bursaria Chlorella virus NC1A Modification methylase CviBIII (CVIBIIIM) CSB-YP318788PCI
CSB-EP318788PCI
CSB-BP318788PCI
CSB-MP318788PCI
CSB-EP318788PCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mating-type P-specific polypeptide Pi (matPi) CSB-YP318789SXU
CSB-EP318789SXU
CSB-BP318789SXU
CSB-MP318789SXU
CSB-EP318789SXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L14, mitochondrial CSB-YP318790TLT
CSB-EP318790TLT
CSB-BP318790TLT
CSB-MP318790TLT
CSB-EP318790TLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adhesin yadA (yadA) CSB-YP318791YAH
CSB-EP318791YAH
CSB-BP318791YAH
CSB-MP318791YAH
CSB-EP318791YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rinderpest virus Fusion glycoprotein F0 (F), partial CSB-YP318792REY
CSB-EP318792REY
CSB-BP318792REY
CSB-MP318792REY
CSB-EP318792REY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Aspartokinase (HOM3), partial CSB-YP318793SVG
CSB-EP318793SVG
CSB-BP318793SVG
CSB-MP318793SVG
CSB-EP318793SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin alpha chain CSB-YP318794TLT
CSB-EP318794TLT
CSB-BP318794TLT
CSB-MP318794TLT
CSB-EP318794TLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP318795HWQ1
CSB-EP318795HWQ1
CSB-BP318795HWQ1
CSB-MP318795HWQ1
CSB-EP318795HWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli sn-glycerol-3-phosphate import ATP-binding protein UgpC (ugpC) CSB-YP318796ENV
CSB-EP318796ENV
CSB-BP318796ENV
CSB-MP318796ENV
CSB-EP318796ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 2 (M), partial CSB-YP318797IEC1
CSB-EP318797IEC1
CSB-BP318797IEC1
CSB-MP318797IEC1
CSB-EP318797IEC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Short tail fiber protein (12), partial CSB-YP318798EDZ
CSB-EP318798EDZ
CSB-BP318798EDZ
CSB-MP318798EDZ
CSB-EP318798EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryphonectria hypovirus 1 Polyprotein p69 CSB-YP318799CTE
CSB-EP318799CTE
CSB-BP318799CTE
CSB-MP318799CTE
CSB-EP318799CTE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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