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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterobacteria phage T4 Valyl--tRNA ligase modifier (vs) CSB-YP320206EDZ
CSB-EP320206EDZ
CSB-BP320206EDZ
CSB-MP320206EDZ
CSB-EP320206EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 10.9 kDa protein in regB-denV intergenic region (y06F) CSB-YP320207EDZ
CSB-EP320207EDZ
CSB-BP320207EDZ
CSB-MP320207EDZ
CSB-EP320207EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Autonomous glycyl radical cofactor (grcA) CSB-YP320208EDZ
CSB-EP320208EDZ
CSB-BP320208EDZ
CSB-MP320208EDZ
CSB-EP320208EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 11.2 kDa protein in segB-ipI intergenic region (y07B) CSB-YP320209EDZ
CSB-EP320209EDZ
CSB-BP320209EDZ
CSB-MP320209EDZ
CSB-EP320209EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 12.4 kDa protein in mobB-Gp55 intergenic region (y03H) CSB-YP320210EDZ
CSB-EP320210EDZ
CSB-BP320210EDZ
CSB-MP320210EDZ
CSB-EP320210EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 7.3 kDa protein in mobB-Gp55 intergenic region (y03F) CSB-YP320211EDZ
CSB-EP320211EDZ
CSB-BP320211EDZ
CSB-MP320211EDZ
CSB-EP320211EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Structural protein of head (20), partial CSB-YP320212EDZ
CSB-EP320212EDZ
CSB-BP320212EDZ
CSB-MP320212EDZ
CSB-EP320212EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Tail-tube assembly protein Gp48 (48) CSB-YP320213EDZ
CSB-EP320213EDZ
CSB-BP320213EDZ
CSB-MP320213EDZ
CSB-EP320213EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysis protein for colicin E5 (lys) CSB-YP320214ENL
CSB-EP320214ENL
CSB-BP320214ENL
CSB-MP320214ENL
CSB-EP320214ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorella protothecoides Cytochrome b6 (petB), partial CSB-YP320215CJG1
CSB-EP320215CJG1
CSB-BP320215CJG1
CSB-MP320215CJG1
CSB-EP320215CJG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Immunoglobulin omega chain (Vpreb2) CSB-YP320216MO
CSB-EP320216MO
CSB-BP320216MO
CSB-MP320216MO
CSB-EP320216MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Glucose-6-phosphate isomerase A (pgiA) CSB-YP320217GFM
CSB-EP320217GFM
CSB-BP320217GFM
CSB-MP320217GFM
CSB-EP320217GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dirofilaria immitis Paramyosin, partial CSB-YP320218DKS
CSB-EP320218DKS
CSB-BP320218DKS
CSB-MP320218DKS
CSB-EP320218DKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. 6-aminohexanoate-cyclic-dimer hydrolase (nylA) CSB-YP320219PVQ
CSB-EP320219PVQ
CSB-BP320219PVQ
CSB-MP320219PVQ
CSB-EP320219PVQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Intrinsic membrane protein pufX (pufX) CSB-YP320220RLF
CSB-EP320220RLF
CSB-BP320220RLF
CSB-MP320220RLF
CSB-EP320220RLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Toxoplasma gondii Dense granule protein 2 (GRA2) CSB-YP320221TOV
CSB-BP320221TOV
CSB-MP320221TOV
CSB-EP320221TOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinapis alba Chalcone synthase 3 (CHS3) CSB-YP320222SZO
CSB-EP320222SZO
CSB-BP320222SZO
CSB-MP320222SZO
CSB-EP320222SZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Protective antigen (pagA), partial CSB-YP320223BQE
CSB-EP320223BQE
CSB-BP320223BQE
CSB-MP320223BQE
CSB-EP320223BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila subobscura Chorion protein S19 (Cp19) CSB-YP320224DML
CSB-EP320224DML
CSB-BP320224DML
CSB-MP320224DML
CSB-EP320224DML-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat SMR1 protein (Vcsa1) CSB-YP320225RA
CSB-EP320225RA
CSB-BP320225RA
CSB-MP320225RA
CSB-EP320225RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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