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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rat Peptidyl-prolyl cis-trans isomerase A (Ppia) CSB-YP320617RA
CSB-EP320617RA
CSB-BP320617RA
CSB-MP320617RA
CSB-EP320617RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma capricolum subsp. capricolum 50S ribosomal protein L22 (rplV) CSB-YP320618MLJ
CSB-EP320618MLJ
CSB-BP320618MLJ
CSB-MP320618MLJ
CSB-EP320618MLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Envelope glycoprotein L (gL) CSB-YP320619HWY
CSB-EP320619HWY
CSB-BP320619HWY
CSB-MP320619HWY
CSB-EP320619HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 DNA helicase/primase complex-associated protein (UL8), partial CSB-YP320620HWY
CSB-EP320620HWY
CSB-BP320620HWY
CSB-MP320620HWY
CSB-EP320620HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Capsid vertex component 1 (CVC1), partial CSB-YP320621HWY
CSB-EP320621HWY
CSB-MP320621HWY
CSB-EP320621HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Tripartite terminase subunit UL28 (UL28), partial CSB-YP320622HWY
CSB-EP320622HWY
CSB-BP320622HWY
CSB-MP320622HWY
CSB-EP320622HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Capsid protein VP26 (UL35) CSB-YP320623HWY
CSB-EP320623HWY
CSB-BP320623HWY
CSB-MP320623HWY
CSB-EP320623HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Virion host shutoff protein (UL41) CSB-YP320624HWY
CSB-EP320624HWY
CSB-BP320624HWY
CSB-MP320624HWY
CSB-EP320624HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Membrane protein UL43 (UL43), partial CSB-YP320625HWY
CSB-EP320625HWY
CSB-BP320625HWY
CSB-MP320625HWY
CSB-EP320625HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sucrose phosphorylase (gtfA) CSB-YP320627FMR
CSB-EP320627FMR
CSB-BP320627FMR
CSB-MP320627FMR
CSB-EP320627FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus keta Gonadotropin subunit beta-2 (cgbb) CSB-YP320628OBG
CSB-EP320628OBG
CSB-BP320628OBG
CSB-MP320628OBG
CSB-EP320628OBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Baboon endogenous virus Envelope glycoprotein (env), partial CSB-YP320630BAB
CSB-EP320630BAB
CSB-BP320630BAB
CSB-MP320630BAB
CSB-EP320630BAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P4 Putative P4-specific DNA primase (Alpha), partial CSB-YP320631EDH
CSB-EP320631EDH
CSB-BP320631EDH
CSB-MP320631EDH
CSB-EP320631EDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 DNA mismatch repair protein MutS (mutS), partial CSB-YP320632SMW
CSB-EP320632SMW
CSB-BP320632SMW
CSB-MP320632SMW
CSB-EP320632SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP320633SMV
CSB-EP320633SMV
CSB-BP320633SMV
CSB-MP320633SMV
CSB-EP320633SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Transcriptional repressor NrdR (nrdR) CSB-YP320634SMW
CSB-EP320634SMW
CSB-BP320634SMW
CSB-MP320634SMW
CSB-EP320634SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate carbamoyltransferase (pyrB) CSB-YP320635SMV
CSB-EP320635SMV
CSB-BP320635SMV
CSB-MP320635SMV
CSB-EP320635SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Putative 2-dehydropantoate 2-reductase (apbA) CSB-YP320636SMW
CSB-EP320636SMW
CSB-BP320636SMW
CSB-MP320636SMW
CSB-EP320636SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptidase T (pepT) CSB-YP320637SMV
CSB-EP320637SMV
CSB-BP320637SMV
CSB-MP320637SMV
CSB-EP320637SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP320638SMW
CSB-EP320638SMW
CSB-BP320638SMW
CSB-MP320638SMW
CSB-EP320638SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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