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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Streptococcus pyogenes serotype M3 30S ribosomal protein S21 (rpsU) CSB-YP319797SMW
CSB-EP319797SMW
CSB-BP319797SMW
CSB-MP319797SMW
CSB-EP319797SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 30S ribosomal protein S4 (rpsD) CSB-YP319798SMW
CSB-EP319798SMW
CSB-BP319798SMW
CSB-MP319798SMW
CSB-EP319798SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 30S ribosomal protein S5 (rpsE) CSB-YP319799SMW
CSB-EP319799SMW
CSB-BP319799SMW
CSB-MP319799SMW
CSB-EP319799SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 30S ribosomal protein S9 (rpsI) CSB-YP319800SMW
CSB-EP319800SMW
CSB-BP319800SMW
CSB-MP319800SMW
CSB-EP319800SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP319801SMW
CSB-EP319801SMW
CSB-BP319801SMW
CSB-MP319801SMW
CSB-EP319801SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease Y (rny), partial CSB-YP319802SMV
CSB-EP319802SMV
CSB-BP319802SMV
CSB-MP319802SMV
CSB-EP319802SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP319803SMV
CSB-EP319803SMV
CSB-BP319803SMV
CSB-MP319803SMV
CSB-EP319803SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase I (rsmI) CSB-YP319804SMV
CSB-EP319804SMV
CSB-BP319804SMV
CSB-MP319804SMV
CSB-EP319804SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Putative Holliday junction resolvase (SPs1795) CSB-YP319805SMW
CSB-EP319805SMW
CSB-BP319805SMW
CSB-MP319805SMW
CSB-EP319805SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zinc-binding protein AdcA (adcA) CSB-YP319806SMV
CSB-EP319806SMV
CSB-BP319806SMV
CSB-MP319806SMV
CSB-EP319806SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Segregation and condensation protein B (scpB) CSB-YP319807SMW
CSB-EP319807SMW
CSB-BP319807SMW
CSB-MP319807SMW
CSB-EP319807SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Probable transcriptional regulatory protein SPs1628 (SPs1628) CSB-YP319808SMW
CSB-EP319808SMW
CSB-BP319808SMW
CSB-MP319808SMW
CSB-EP319808SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Streptolysin O (slo), partial CSB-YP319809SMW
CSB-EP319809SMW
CSB-BP319809SMW
CSB-MP319809SMW
CSB-EP319809SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA gyrase subunit A (gyrA), partial CSB-YP319810SMV
CSB-EP319810SMV
CSB-BP319810SMV
CSB-MP319810SMV
CSB-EP319810SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Exotoxin type G (speG) CSB-YP319811SMW
CSB-EP319811SMW
CSB-BP319811SMW
CSB-MP319811SMW
CSB-EP319811SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized RNA methyltransferase SpyM3_1299 (SpyM3_1299) CSB-YP319812SMV
CSB-EP319812SMV
CSB-BP319812SMV
CSB-MP319812SMV
CSB-EP319812SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA modification GTPase MnmE (mnmE) CSB-YP319813SMV
CSB-EP319813SMV
CSB-BP319813SMV
CSB-MP319813SMV
CSB-EP319813SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate--tRNA ligase (aspS), partial CSB-YP319814SMV
CSB-EP319814SMV
CSB-BP319814SMV
CSB-MP319814SMV
CSB-EP319814SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Cysteine--tRNA ligase (cysS) CSB-YP319815SMW
CSB-EP319815SMW
CSB-BP319815SMW
CSB-MP319815SMW
CSB-EP319815SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidine--tRNA ligase (hisS) CSB-YP319816SMV
CSB-EP319816SMV
CSB-BP319816SMV
CSB-MP319816SMV
CSB-EP319816SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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