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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dhori virus Nucleoprotein (P5) CSB-YP320002DDL
CSB-EP320002DDL
CSB-BP320002DDL
CSB-MP320002DDL
CSB-EP320002DDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus Photosystem II manganese-stabilizing polypeptide (psbO) CSB-YP320003FPY
CSB-EP320003FPY
CSB-BP320003FPY
CSB-MP320003FPY
CSB-EP320003FPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Neutrophil cationic peptide 1 type A CSB-YP320004GU
CSB-EP320004GU
CSB-BP320004GU
CSB-MP320004GU
CSB-EP320004GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium difficile Chloramphenicol acetyltransferase (catD) CSB-YP320005DUN
CSB-BP320005DUN
CSB-MP320005DUN
CSB-EP320005DUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Hemoglobin subunit beta-2 CSB-YP320006RA
CSB-EP320006RA
CSB-BP320006RA
CSB-MP320006RA
CSB-EP320006RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arbacia punctulata Resact receptor, partial CSB-YP320007ANL
CSB-EP320007ANL
CSB-BP320007ANL
CSB-MP320007ANL
CSB-EP320007ANL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Barstar CSB-YP320008BQB
CSB-BP320008BQB
CSB-MP320008BQB
CSB-EP320008BQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Methylenomycin A resistance protein (mmr), partial CSB-YP320009FOB
CSB-EP320009FOB
CSB-BP320009FOB
CSB-MP320009FOB
CSB-EP320009FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-fucose-proton symporter (fucP), partial CSB-YP320010ENV
CSB-EP320010ENV
CSB-BP320010ENV
CSB-MP320010ENV
CSB-EP320010ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methyl-coenzyme M reductase subunit alpha (mcrA), partial CSB-YP320011MSD
CSB-EP320011MSD
CSB-BP320011MSD
CSB-MP320011MSD
CSB-EP320011MSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methyl-coenzyme M reductase subunit gamma (mcrG) CSB-YP320012MSD
CSB-EP320012MSD
CSB-BP320012MSD
CSB-MP320012MSD
CSB-EP320012MSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidaminococcus fermentans Activator of (R)-2-hydroxyglutaryl-CoA dehydratase CSB-YP320013AWA
CSB-EP320013AWA
CSB-BP320013AWA
CSB-MP320013AWA
CSB-EP320013AWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Major urinary protein 5 (Mup5) CSB-YP320014MO
CSB-EP320014MO
CSB-BP320014MO
CSB-MP320014MO
CSB-EP320014MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinapis alba Oxygen-evolving enhancer protein 2, chloroplastic (PSBP) CSB-YP320015SZO
CSB-EP320015SZO
CSB-BP320015SZO
CSB-MP320015SZO
CSB-EP320015SZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Nitrate reductase [NADH] 1 (NIA1), partial CSB-YP320016NHE
CSB-EP320016NHE
CSB-BP320016NHE
CSB-MP320016NHE
CSB-EP320016NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (PPP2CB) CSB-YP320017RB
CSB-EP320017RB
CSB-BP320017RB
CSB-MP320017RB
CSB-EP320017RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe GTP-binding protein ypt1 (ypt1) CSB-YP320018SXV
CSB-EP320018SXV
CSB-BP320018SXV
CSB-MP320018SXV
CSB-EP320018SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Non-histone chromosomal protein 6A (NHP6A) CSB-YP320019SVG
CSB-EP320019SVG
CSB-BP320019SVG
CSB-MP320019SVG
CSB-EP320019SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Quinate permease (qa-y), partial CSB-YP320020NHA
CSB-EP320020NHA
CSB-BP320020NHA
CSB-MP320020NHA
CSB-EP320020NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Alpha-amylase/trypsin inhibitor CMd (IAT3) CSB-YP320021HWQ
CSB-EP320021HWQ
CSB-BP320021HWQ
CSB-MP320021HWQ
CSB-EP320021HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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