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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Laticauda laticaudata Short neurotoxin C CSB-YP320759LDB
CSB-EP320759LDB
CSB-BP320759LDB
CSB-MP320759LDB
CSB-EP320759LDB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus X Coat protein CSB-YP320760PRG
CSB-EP320760PRG
CSB-BP320760PRG
CSB-MP320760PRG
CSB-EP320760PRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Endoglucanase/exoglucanase B (celB), partial CSB-YP320761CAM
CSB-EP320761CAM
CSB-BP320761CAM
CSB-MP320761CAM
CSB-EP320761CAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage PRD1 DNA-directed DNA polymerase (I), partial CSB-YP320762EDO
CSB-EP320762EDO
CSB-BP320762EDO
CSB-MP320762EDO
CSB-EP320762EDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type I restriction enzyme EcoR124II M protein (hsdM) CSB-YP320763ENL
CSB-EP320763ENL
CSB-BP320763ENL
CSB-MP320763ENL
CSB-EP320763ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin alpha chain CSB-YP320764LEO
CSB-EP320764LEO
CSB-BP320764LEO
CSB-MP320764LEO
CSB-EP320764LEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Glycine-rich cell wall structural protein 1.8 CSB-YP320765EUN
CSB-EP320765EUN
CSB-BP320765EUN
CSB-MP320765EUN
CSB-EP320765EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Sodium/proline symporter (putP), partial CSB-YP320766SXB
CSB-EP320766SXB
CSB-BP320766SXB
CSB-MP320766SXB
CSB-EP320766SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium sp. Uncharacterized plasmid pDG1 103 kDa protein, partial CSB-YP320767DDV
CSB-EP320767DDV
CSB-BP320767DDV
CSB-MP320767DDV
CSB-EP320767DDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine coronavirus Nucleoprotein (N) CSB-YP320768BJK
CSB-BP320768BJK
CSB-MP320768BJK
CSB-EP320768BJK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine/threonine-protein kinase-transforming protein Rmil (V-RMIL) CSB-YP320769ASX
CSB-EP320769ASX
CSB-BP320769ASX
CSB-MP320769ASX
CSB-EP320769ASX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Beta-amylase (BMY1) CSB-YP320770GGV
CSB-EP320770GGV
CSB-BP320770GGV
CSB-MP320770GGV
CSB-EP320770GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus F serotype 40 E1B protein, large T-antigen CSB-YP320771HIR
CSB-EP320771HIR
CSB-BP320771HIR
CSB-MP320771HIR
CSB-EP320771HIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysostaphin (lss) CSB-YP320772SLK
CSB-EP320772SLK
CSB-BP320772SLK
CSB-MP320772SLK
CSB-EP320772SLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Virulence plasmid protein pGP6-D CSB-YP320773DSA
CSB-EP320773DSA
CSB-BP320773DSA
CSB-MP320773DSA
CSB-EP320773DSA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Larvicidal toxin 51 kDa protein (binB) CSB-YP320774LRU
CSB-EP320774LRU
CSB-BP320774LRU
CSB-MP320774LRU
CSB-EP320774LRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small, acid-soluble spore protein C2 (SASP-C2) CSB-YP320775BQV
CSB-EP320775BQV
CSB-BP320775BQV
CSB-MP320775BQV
CSB-EP320775BQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Nitrogen assimilation regulatory protein (ntrC) CSB-YP320776RKU
CSB-EP320776RKU
CSB-BP320776RKU
CSB-MP320776RKU
CSB-EP320776RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Probable DNA-directed RNA polymerase, partial CSB-YP320777ZAX
CSB-EP320777ZAX
CSB-BP320777ZAX
CSB-MP320777ZAX
CSB-EP320777ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Gluconate operon transcriptional repressor (gntR) CSB-YP320778BRJ
CSB-EP320778BRJ
CSB-BP320778BRJ
CSB-MP320778BRJ
CSB-EP320778BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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