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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Human adenovirus C serotype 2 Late L2 mu core protein (PX) CSB-YP320349HIK
CSB-EP320349HIK
CSB-BP320349HIK
CSB-MP320349HIK
CSB-EP320349HIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Chlorophyll a-b binding protein 1A, chloroplastic (CAB1A), partial CSB-YP320350SZY1
CSB-EP320350SZY1
CSB-BP320350SZY1
CSB-MP320350SZY1
CSB-EP320350SZY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Chlorophyll a-b binding protein 3A, chloroplastic (CAB3A), partial CSB-YP320351SZY1
CSB-EP320351SZY1
CSB-BP320351SZY1
CSB-MP320351SZY1
CSB-EP320351SZY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Chlorophyll a-b binding protein 5, chloroplastic (CAB5), partial CSB-YP320352SZY1
CSB-EP320352SZY1
CSB-BP320352SZY1
CSB-MP320352SZY1
CSB-EP320352SZY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 22 kDa protein in overamplified macronuclear DNA POB4 CSB-YP320353SQX
CSB-EP320353SQX
CSB-BP320353SQX
CSB-MP320353SQX
CSB-EP320353SQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida boidinii Putative peroxiredoxin-B (PMPB) CSB-YP320354CZG
CSB-EP320354CZG
CSB-BP320354CZG
CSB-MP320354CZG
CSB-EP320354CZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Manduca sexta Arylphorin subunit beta, partial CSB-YP320355MPV
CSB-EP320355MPV
CSB-BP320355MPV
CSB-MP320355MPV
CSB-EP320355MPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ADP-ribosyl-[dinitrogen reductase] glycohydrolase (draG) CSB-YP320356RMA
CSB-EP320356RMA
CSB-BP320356RMA
CSB-MP320356RMA
CSB-EP320356RMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Carboxypeptidase Y inhibitor (TFS1) CSB-YP320357SVG
CSB-EP320357SVG
CSB-BP320357SVG
CSB-MP320357SVG
CSB-EP320357SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein fixW (fixW) CSB-YP320358RKN
CSB-EP320358RKN
CSB-BP320358RKN
CSB-MP320358RKN
CSB-EP320358RKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable glutamine--tRNA ligase (glnS), partial CSB-YP320359DKK
CSB-EP320359DKK
CSB-BP320359DKK
CSB-MP320359DKK
CSB-EP320359DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia solanacearum Isopentenyl transferase (tzs) CSB-YP320360RJJ
CSB-EP320360RJJ
CSB-BP320360RJJ
CSB-MP320360RJJ
CSB-EP320360RJJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kunjin virus Genome polyprotein, partial CSB-YP320361KAT
CSB-EP320361KAT
CSB-BP320361KAT
CSB-MP320361KAT
CSB-EP320361KAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Helicase/primase complex protein (56), partial CSB-YP320362SAX
CSB-EP320362SAX
CSB-BP320362SAX
CSB-MP320362SAX
CSB-EP320362SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human spumaretrovirus Protein Bel-1 (bel1) CSB-YP320363HQF
CSB-EP320363HQF
CSB-BP320363HQF
CSB-MP320363HQF
CSB-EP320363HQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein N2 (N2L) CSB-YP320364VAI
CSB-EP320364VAI
CSB-BP320364VAI
CSB-MP320364VAI
CSB-EP320364VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Uncharacterized protein FPV249 (FPV249) CSB-YP320365FEY
CSB-EP320365FEY
CSB-BP320365FEY
CSB-MP320365FEY
CSB-EP320365FEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fowlpox virus Putative protein FPV235 (FPV235) CSB-YP320366FEY
CSB-EP320366FEY
CSB-BP320366FEY
CSB-MP320366FEY
CSB-EP320366FEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine herpesvirus 1.1 Glycoprotein GIII (GIII), partial CSB-YP320367BJS
CSB-EP320367BJS
CSB-BP320367BJS
CSB-MP320367BJS
CSB-EP320367BJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Modification methylase TaqI (taqIM) CSB-YP320368TNN
CSB-EP320368TNN
CSB-BP320368TNN
CSB-MP320368TNN
CSB-EP320368TNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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