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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Tryptophan biosynthesis protein TrpCF (trpC) CSB-YP322084VFE
CSB-EP322084VFE
CSB-BP322084VFE
CSB-MP322084VFE
CSB-EP322084VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthranilate synthase component II (trpG) CSB-YP322085VFE
CSB-EP322085VFE
CSB-BP322085VFE
CSB-MP322085VFE
CSB-EP322085VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orgyia pseudotsugata multicapsid polyhedrosis virus Trans-activating transcriptional regulatory protein (IE1), partial CSB-YP322086OEU
CSB-EP322086OEU
CSB-BP322086OEU
CSB-MP322086OEU
CSB-EP322086OEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peanut stunt virus Movement protein (ORF3a) CSB-YP322087PDZ
CSB-EP322087PDZ
CSB-BP322087PDZ
CSB-MP322087PDZ
CSB-EP322087PDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maize rough dwarf virus Probable non-structural 41.0 kDa protein (S6) CSB-YP322088MBI
CSB-EP322088MBI
CSB-BP322088MBI
CSB-MP322088MBI
CSB-EP322088MBI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Discopyge ommata Ras-like GTP-binding protein O-RHO CSB-YP322089DES
CSB-EP322089DES
CSB-BP322089DES
CSB-MP322089DES
CSB-EP322089DES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protein ras-1 (ras-1) CSB-YP322090NHA
CSB-EP322090NHA
CSB-BP322090NHA
CSB-MP322090NHA
CSB-EP322090NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytophthora infestans Actin-2 (ACTB) CSB-YP322091PJO
CSB-EP322091PJO
CSB-BP322091PJO
CSB-MP322091PJO
CSB-EP322091PJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Clathrin heavy chain (CHC1), partial CSB-YP322092SVG
CSB-EP322092SVG
CSB-BP322092SVG
CSB-MP322092SVG
CSB-EP322092SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerases I, II, and III subunit RPABC5 (RPB10) CSB-YP322093SVG
CSB-EP322093SVG
CSB-BP322093SVG
CSB-MP322093SVG
CSB-EP322093SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanamide hydratase (CAH) CSB-YP322094MMP
CSB-EP322094MMP
CSB-BP322094MMP
CSB-MP322094MMP
CSB-EP322094MMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis D-xylulose reductase (XYL2) CSB-YP322095SXM
CSB-EP322095SXM
CSB-BP322095SXM
CSB-MP322095SXM
CSB-EP322095SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein MSN1 (MSN1) CSB-YP322096SVG
CSB-EP322096SVG
CSB-BP322096SVG
CSB-MP322096SVG
CSB-EP322096SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication protein E1 (E1), partial CSB-YP322097HOD
CSB-EP322097HOD
CSB-BP322097HOD
CSB-MP322097HOD
CSB-EP322097HOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhesus papillomavirus type 1 Regulatory protein E2 (E2) CSB-YP322098RCO
CSB-EP322098RCO
CSB-BP322098RCO
CSB-MP322098RCO
CSB-EP322098RCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein E6 (E6) CSB-YP322099HOD
CSB-EP322099HOD
CSB-BP322099HOD
CSB-MP322099HOD
CSB-EP322099HOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhesus papillomavirus type 1 Protein E7 (E7) CSB-YP322100RCO
CSB-EP322100RCO
CSB-BP322100RCO
CSB-MP322100RCO
CSB-EP322100RCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simulium iridescent virus Major capsid protein (MCP) CSB-YP322101SHW
CSB-EP322101SHW
CSB-BP322101SHW
CSB-MP322101SHW
CSB-EP322101SHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine respiratory syncytial virus Fusion glycoprotein F0 (F), partial CSB-YP322102BKU
CSB-EP322102BKU
CSB-BP322102BKU
CSB-MP322102BKU
CSB-EP322102BKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Movement protein TGBp3 (ORF4), partial CSB-YP322103FCN
CSB-EP322103FCN
CSB-BP322103FCN
CSB-MP322103FCN
CSB-EP322103FCN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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