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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mopeia virus Pre-glycoprotein polyprotein GP complex (GPC), partial CSB-YP321673MHY1
CSB-EP321673MHY1
CSB-BP321673MHY1
CSB-EP321673MHY1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Coat protein TP3 CSB-YP321674TDO
CSB-EP321674TDO
CSB-BP321674TDO
CSB-MP321674TDO
CSB-EP321674TDO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 6.9 kDa protein CSB-YP321675TDO
CSB-EP321675TDO
CSB-BP321675TDO
CSB-MP321675TDO
CSB-EP321675TDO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 10.0 kDa protein CSB-YP321676TDO
CSB-EP321676TDO
CSB-BP321676TDO
CSB-MP321676TDO
CSB-EP321676TDO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 15.4 kDa protein CSB-YP321677TDO
CSB-EP321677TDO
CSB-BP321677TDO
CSB-MP321677TDO
CSB-EP321677TDO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 16.5 kDa protein CSB-YP321678TDO
CSB-EP321678TDO
CSB-BP321678TDO
CSB-MP321678TDO
CSB-EP321678TDO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 6.2 kDa protein CSB-YP321679TDO
CSB-EP321679TDO
CSB-BP321679TDO
CSB-MP321679TDO
CSB-EP321679TDO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus tenax virus 1 Uncharacterized 38.7 kDa protein CSB-YP321680TDO
CSB-EP321680TDO
CSB-BP321680TDO
CSB-MP321680TDO
CSB-EP321680TDO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Formate hydrogenlyase transcriptional activator (fhlA) CSB-YP321681ENV
CSB-EP321681ENV
CSB-BP321681ENV
CSB-MP321681ENV
CSB-EP321681ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein sex-lethal (Sxl) CSB-YP321682DLU
CSB-EP321682DLU
CSB-BP321682DLU
CSB-MP321682DLU
CSB-EP321682DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 28 kDa antigen (ML0091) CSB-YP321683MVN
CSB-EP321683MVN
CSB-BP321683MVN
CSB-MP321683MVN
CSB-EP321683MVN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus phage Cp-7 Lysozyme (CPL7) CSB-YP321684SMN
CSB-EP321684SMN
CSB-BP321684SMN
CSB-MP321684SMN
CSB-EP321684SMN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mating-type protein A-1 (mtA-1) CSB-YP321685NHA
CSB-EP321685NHA
CSB-BP321685NHA
CSB-MP321685NHA
CSB-EP321685NHA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes M protein, serotype 12 (emm12), partial CSB-YP321686SMR
CSB-EP321686SMR
CSB-BP321686SMR
CSB-MP321686SMR
CSB-EP321686SMR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea 6.7 kDa chloroplast outer envelope membrane protein, partial CSB-YP321687FKI
CSB-EP321687FKI
CSB-BP321687FKI
CSB-MP321687FKI
CSB-EP321687FKI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eubacterium sp. Bile acid-CoA hydrolase (baiF) CSB-YP321688EHF
CSB-EP321688EHF
CSB-BP321688EHF
CSB-MP321688EHF
CSB-EP321688EHF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase sigma-B factor (sigB) CSB-YP321689MXA
CSB-EP321689MXA
CSB-BP321689MXA
CSB-MP321689MXA
CSB-EP321689MXA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Probable glutamate receptor (KBP), partial CSB-YP321690CH1
CSB-EP321690CH1
CSB-BP321690CH1
CSB-MP321690CH1
CSB-EP321690CH1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrullus lanatus Malate dehydrogenase, glyoxysomal CSB-YP321691CKU
CSB-EP321691CKU
CSB-BP321691CKU
CSB-MP321691CKU
CSB-EP321691CKU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Formimidoylglutamase (hutG) CSB-YP321692EKN
CSB-EP321692EKN
CSB-BP321692EKN
CSB-MP321692EKN
CSB-EP321692EKN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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