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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schistosoma mansoni Calcium-binding protein CSB-YP320246SXQ
CSB-EP320246SXQ
CSB-BP320246SXQ
CSB-MP320246SXQ
CSB-EP320246SXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Heptapoietin A light chain CSB-YP320247RB
CSB-EP320247RB
CSB-BP320247RB
CSB-MP320247RB
CSB-EP320247RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S12 (rpsL) CSB-YP320248FKP
CSB-EP320248FKP
CSB-BP320248FKP
CSB-MP320248FKP
CSB-EP320248FKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lumbricus terrestris Extracellular globin-4 CSB-YP320249LJN
CSB-EP320249LJN
CSB-BP320249LJN
CSB-MP320249LJN
CSB-EP320249LJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicular stomatitis New Jersey virus RNA-directed RNA polymerase L (L), partial CSB-YP320250VBK
CSB-EP320250VBK
CSB-BP320250VBK
CSB-MP320250VBK
CSB-EP320250VBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orgyia pseudotsugata multicapsid polyhedrosis virus Major envelope glycoprotein (GP64), partial CSB-YP320251OEU1
CSB-EP320251OEU1
CSB-BP320251OEU1
CSB-MP320251OEU1
CSB-EP320251OEU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracentrotus lividus Histone H2A CSB-YP320252ERM
CSB-EP320252ERM
CSB-BP320252ERM
CSB-MP320252ERM
CSB-EP320252ERM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Protochlorophyllide reductase A, chloroplastic (PORA) CSB-YP320254HWQ
CSB-EP320254HWQ
CSB-BP320254HWQ
CSB-MP320254HWQ
CSB-EP320254HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mobilization protein mbeC (mbeC) CSB-YP320255ENL
CSB-EP320255ENL
CSB-BP320255ENL
CSB-MP320255ENL
CSB-EP320255ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mobilization protein mbeB (mbeB) CSB-YP320256ENL
CSB-EP320256ENL
CSB-BP320256ENL
CSB-MP320256ENL
CSB-EP320256ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Protein ENDO16 (ENDO16) CSB-YP320257FOT
CSB-EP320257FOT
CSB-BP320257FOT
CSB-MP320257FOT
CSB-EP320257FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Mannosyl-D-glycerate transport/metabolism system repressor mngR (mngR) CSB-YP320258ENV
CSB-EP320258ENV
CSB-BP320258ENV
CSB-MP320258ENV
CSB-EP320258ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Skin granule protein (sgp) CSB-YP320259XBE
CSB-EP320259XBE
CSB-BP320259XBE
CSB-MP320259XBE
CSB-EP320259XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Serine/threonine-protein phosphatase PP1-1 (dis2) CSB-YP320260SXV
CSB-EP320260SXV
CSB-BP320260SXV
CSB-MP320260SXV
CSB-EP320260SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Sucrose synthase (SS), partial CSB-YP320261GGV
CSB-EP320261GGV
CSB-BP320261GGV
CSB-MP320261GGV
CSB-EP320261GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Chromatin assembly factor 1 subunit p50 (MSI1) CSB-YP320262SVG
CSB-EP320262SVG
CSB-BP320262SVG
CSB-MP320262SVG
CSB-EP320262SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-lactate dehydrogenase (ldh) CSB-YP320263TNN
CSB-EP320263TNN
CSB-BP320263TNN
CSB-MP320263TNN
CSB-EP320263TNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Beta-hexosaminidase subunit A1 (hexa1), partial CSB-YP320264DKK
CSB-EP320264DKK
CSB-BP320264DKK
CSB-MP320264DKK
CSB-EP320264DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Salivary glue protein Sgs-3 (Sgs3) CSB-YP320265DMJ
CSB-EP320265DMJ
CSB-BP320265DMJ
CSB-MP320265DMJ
CSB-EP320265DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alteromonas atlantica Beta-agarase (agrA) CSB-YP320266AHZ
CSB-EP320266AHZ
CSB-BP320266AHZ
CSB-MP320266AHZ
CSB-EP320266AHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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