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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Tetracycline resistance determinant (tetB), partial CSB-YP320390SPJ
CSB-EP320390SPJ
CSB-BP320390SPJ
CSB-MP320390SPJ
CSB-EP320390SPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit gamma (rpoC1), partial CSB-YP320391NEF
CSB-EP320391NEF
CSB-BP320391NEF
CSB-MP320391NEF
CSB-EP320391NEF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rice dwarf virus Putative mRNA-capping enzyme P5, partial CSB-YP320392REN
CSB-EP320392REN
CSB-BP320392REN
CSB-MP320392REN
CSB-EP320392REN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tabanus atratus Adipokinetic hormone CSB-YP320395TAA
CSB-EP320395TAA
CSB-BP320395TAA
CSB-MP320395TAA
CSB-EP320395TAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Cyclic AMP-inducible protein BP74 (cigA), partial CSB-YP320396DKK
CSB-EP320396DKK
CSB-BP320396DKK
CSB-MP320396DKK
CSB-EP320396DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Glutelin type-B 4 (GLUB4) CSB-YP320397OFG
CSB-BP320397OFG
CSB-MP320397OFG
CSB-EP320397OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Newcastle disease virus Fusion glycoprotein F0 (F), partial CSB-YP320398NCY
CSB-EP320398NCY
CSB-BP320398NCY
CSB-MP320398NCY
CSB-EP320398NCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Photosystem I reaction center subunit psaK, chloroplastic (PSAK), partial CSB-YP320399FKI
CSB-EP320399FKI
CSB-BP320399FKI
CSB-MP320399FKI
CSB-EP320399FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Astasia longa Elongation factor Tu, plastid (tufA) CSB-YP320400APY
CSB-EP320400APY
CSB-BP320400APY
CSB-MP320400APY
CSB-EP320400APY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan synthase alpha chain (trpA) CSB-YP320401MSD
CSB-EP320401MSD
CSB-BP320401MSD
CSB-MP320401MSD
CSB-EP320401MSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Tubulin alpha-1 chain (TUBA1) CSB-YP320402ZAX
CSB-EP320402ZAX
CSB-BP320402ZAX
CSB-MP320402ZAX
CSB-EP320402ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Glutamine synthetase, chloroplastic (GLN2) CSB-YP320404OFG
CSB-EP320404OFG
CSB-BP320404OFG
CSB-MP320404OFG
CSB-EP320404OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Photosystem Q (B) protein, partial CSB-YP320405SSO1
CSB-EP320405SSO1
CSB-BP320405SSO1
CSB-MP320405SSO1
CSB-EP320405SSO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ascaris suum Cecropin-P1 (ASCEC-1) CSB-YP320406DOS
CSB-EP320406DOS
CSB-BP320406DOS
CSB-MP320406DOS
CSB-EP320406DOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Manduca sexta Bactericidin B-5P CSB-YP320407MPV
CSB-EP320407MPV
CSB-BP320407MPV
CSB-MP320407MPV
CSB-EP320407MPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Dual specificity protein kinase YAK1 (YAK1), partial CSB-YP320409SVG
CSB-EP320409SVG
CSB-BP320409SVG
CSB-MP320409SVG
CSB-EP320409SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorghum bicolor Kafirin PGK1 CSB-YP320410FJS
CSB-EP320410FJS
CSB-BP320410FJS
CSB-MP320410FJS
CSB-EP320410FJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 6-phospho-beta-galactosidase (lacG) CSB-YP320411LMH
CSB-EP320411LMH
CSB-BP320411LMH
CSB-MP320411LMH
CSB-EP320411LMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Membrane antigen containing repeating peptides CSB-YP320412LOG
CSB-EP320412LOG
CSB-BP320412LOG
CSB-MP320412LOG
CSB-EP320412LOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Mini-circle uncharacterized 12.9 kDa protein (SCO1605) CSB-YP320413FOB
CSB-EP320413FOB
CSB-BP320413FOB
CSB-MP320413FOB
CSB-EP320413FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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