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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ganoderma lucidum Immunomodulatory protein Ling Zhi-8 CSB-BP320455GAW
CSB-MP320455GAW
CSB-EP320455GAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthranilate synthase component 1 (trpE) CSB-YP320456CMO
CSB-EP320456CMO
CSB-BP320456CMO
CSB-MP320456CMO
CSB-EP320456CMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Olisthodiscus luteus Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP320457OBA
CSB-EP320457OBA
CSB-BP320457OBA
CSB-MP320457OBA
CSB-EP320457OBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NH (3)-dependent NAD (+) synthetase CSB-YP320458SMV
CSB-EP320458SMV
CSB-BP320458SMV
CSB-MP320458SMV
CSB-EP320458SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucosamine-6-phosphate deaminase (nagB) CSB-YP320459SMV
CSB-EP320459SMV
CSB-BP320459SMV
CSB-MP320459SMV
CSB-EP320459SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Membrane protein insertase YidC 2 (yidC2), partial CSB-YP320461SMV1
CSB-EP320461SMV1
CSB-BP320461SMV1
CSB-MP320461SMV1
CSB-EP320461SMV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrrolidone-carboxylate peptidase (pcp) CSB-YP320462SMV
CSB-EP320462SMV
CSB-BP320462SMV
CSB-MP320462SMV
CSB-EP320462SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP320463SMW
CSB-EP320463SMW
CSB-BP320463SMW
CSB-MP320463SMW
CSB-EP320463SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate 5-kinase (proB) CSB-YP320464SMV
CSB-EP320464SMV
CSB-BP320464SMV
CSB-MP320464SMV
CSB-EP320464SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protease HtpX homolog (htpX), partial CSB-YP320465SMV1
CSB-EP320465SMV1
CSB-BP320465SMV1
CSB-MP320465SMV1
CSB-EP320465SMV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptidyl-tRNA hydrolase (pth) CSB-YP320466SMV
CSB-EP320466SMV
CSB-BP320466SMV
CSB-MP320466SMV
CSB-EP320466SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP320467SMV
CSB-EP320467SMV
CSB-BP320467SMV
CSB-MP320467SMV
CSB-EP320467SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 DNA repair protein RadA homolog (radA) CSB-YP320468SMW
CSB-EP320468SMW
CSB-BP320468SMW
CSB-MP320468SMW
CSB-EP320468SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 DNA replication and repair protein recF (recF) CSB-YP320469SMW
CSB-EP320469SMW
CSB-BP320469SMW
CSB-MP320469SMW
CSB-EP320469SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L11 (rplK) CSB-YP320470SMV
CSB-EP320470SMV
CSB-BP320470SMV
CSB-MP320470SMV
CSB-EP320470SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L17 (rplQ) CSB-YP320471SMV
CSB-EP320471SMV
CSB-BP320471SMV
CSB-MP320471SMV
CSB-EP320471SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L20 (rplT) CSB-YP320472SMV
CSB-EP320472SMV
CSB-BP320472SMV
CSB-MP320472SMV
CSB-EP320472SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L23 (rplW) CSB-YP320473SMV
CSB-EP320473SMV
CSB-BP320473SMV
CSB-MP320473SMV
CSB-EP320473SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L28 (rpmB) CSB-YP320474SMV
CSB-EP320474SMV
CSB-BP320474SMV
CSB-MP320474SMV
CSB-EP320474SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 50S ribosomal protein L31 type B (rpmE2) CSB-YP320475SMW
CSB-EP320475SMW
CSB-BP320475SMW
CSB-MP320475SMW
CSB-EP320475SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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