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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schistosoma mansoni Female-specific protein 800 CSB-YP322287SXQ
CSB-EP322287SXQ
CSB-BP322287SXQ
CSB-MP322287SXQ
CSB-EP322287SXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clover yellow mosaic virus Movement protein TGBp3 (ORF4), partial CSB-YP322288CMQ
CSB-EP322288CMQ
CSB-BP322288CMQ
CSB-MP322288CMQ
CSB-EP322288CMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Movement protein (ORF3a) CSB-YP322289CUA
CSB-EP322289CUA
CSB-BP322289CUA
CSB-MP322289CUA
CSB-EP322289CUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Insulin-like growth factor I-A (igf1-a) CSB-YP322290XBE
CSB-EP322290XBE
CSB-BP322290XBE
CSB-MP322290XBE
CSB-EP322290XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP322291IFN
CSB-EP322291IFN
CSB-BP322291IFN
CSB-MP322291IFN
CSB-EP322291IFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage phi29 Early protein GP16.5 (16.5) CSB-YP322292BBC
CSB-EP322292BBC
CSB-BP322292BBC
CSB-MP322292BBC
CSB-EP322292BBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Anaphase-promoting complex subunit CDC23 (CDC23), partial CSB-YP322293SVG
CSB-EP322293SVG
CSB-BP322293SVG
CSB-MP322293SVG
CSB-EP322293SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Seminal vesicle protein SVP-2 CSB-YP322294GU
CSB-EP322294GU
CSB-BP322294GU
CSB-MP322294GU
CSB-EP322294GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetracenomycin C polyketide putative beta-ketoacyl synthase 2 (tcmL) CSB-YP322295SOG
CSB-EP322295SOG
CSB-BP322295SOG
CSB-MP322295SOG
CSB-EP322295SOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial outer membrane protein OM45 (OM45), partial CSB-YP322296SVG
CSB-EP322296SVG
CSB-BP322296SVG
CSB-MP322296SVG
CSB-EP322296SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nodulation protein D (nodD) CSB-YP322297RKN
CSB-EP322297RKN
CSB-BP322297RKN
CSB-MP322297RKN
CSB-EP322297RKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Virulence sensor protein BvgS (bvgS), partial CSB-YP322298BUA
CSB-EP322298BUA
CSB-BP322298BUA
CSB-MP322298BUA
CSB-EP322298BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halocynthia roretzi Trypsin inhibitor CSB-YP322299HTQ
CSB-EP322299HTQ
CSB-BP322299HTQ
CSB-MP322299HTQ
CSB-EP322299HTQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nucleoprotein (N) CSB-YP322300MBE
CSB-EP322300MBE
CSB-BP322300MBE
CSB-MP322300MBE
CSB-EP322300MBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Tyrosine-protein phosphatase Lar (Lar), partial CSB-YP322301DLU
CSB-EP322301DLU
CSB-BP322301DLU
CSB-MP322301DLU
CSB-EP322301DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian virus 5 Matrix protein (M) CSB-YP322302SHU
CSB-EP322302SHU
CSB-BP322302SHU
CSB-MP322302SHU
CSB-EP322302SHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae General transcriptional corepressor TUP1 (TUP1), partial CSB-YP322303SVG
CSB-EP322303SVG
CSB-BP322303SVG
CSB-MP322303SVG
CSB-EP322303SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable transcriptional regulator phnF (phnF) CSB-YP322304ENV
CSB-EP322304ENV
CSB-BP322304ENV
CSB-MP322304ENV
CSB-EP322304ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yjdA (yjdA) CSB-YP322305ENV
CSB-EP322305ENV
CSB-BP322305ENV
CSB-MP322305ENV
CSB-EP322305ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Transcript termination protein A18 (VACWR138) CSB-YP322306VAI
CSB-EP322306VAI
CSB-BP322306VAI
CSB-MP322306VAI
CSB-EP322306VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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