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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pisum sativum Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic (GAPA) CSB-YP321026EWE
CSB-EP321026EWE
CSB-BP321026EWE
CSB-MP321026EWE
CSB-EP321026EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar membrane protein PEP5 (PEP5), partial CSB-YP321027SVG
CSB-EP321027SVG
CSB-BP321027SVG
CSB-MP321027SVG
CSB-EP321027SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flock house virus Capsid protein alpha (alpha) CSB-YP321028FCA
CSB-EP321028FCA
CSB-BP321028FCA
CSB-MP321028FCA
CSB-EP321028FCA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 30S ribosomal protein S17 (rpsQ) CSB-YP321029BRJ
CSB-EP321029BRJ
CSB-BP321029BRJ
CSB-MP321029BRJ
CSB-EP321029BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP321030DWY
CSB-EP321030DWY
CSB-BP321030DWY
CSB-MP321030DWY
CSB-EP321030DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Peptidyl-glycine alpha-amidating monooxygenase B (pam-b), partial CSB-YP321031XBE
CSB-EP321031XBE
CSB-BP321031XBE
CSB-MP321031XBE
CSB-EP321031XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chimpanzee hepatitis B virus Protein P (P), partial CSB-YP321032CIJ
CSB-EP321032CIJ
CSB-BP321032CIJ
CSB-MP321032CIJ
CSB-EP321032CIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Woodchuck hepatitis B virus Large envelope protein (S), partial CSB-YP321034WAV1
CSB-EP321034WAV1
CSB-BP321034WAV1
CSB-MP321034WAV1
CSB-EP321034WAV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Woodchuck hepatitis B virus Protein X (X) CSB-YP321035WAV
CSB-EP321035WAV
CSB-BP321035WAV
CSB-MP321035WAV
CSB-EP321035WAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homoserine O-acetyltransferase (MET2), partial CSB-YP321036APE
CSB-EP321036APE
CSB-BP321036APE
CSB-MP321036APE
CSB-EP321036APE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine parvovirus type 2 Capsid protein VP1, partial CSB-YP321037CCS
CSB-EP321037CCS
CSB-BP321037CCS
CSB-MP321037CCS
CSB-EP321037CCS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis B virus genotype C subtype adr Large envelope protein (S), partial CSB-YP321038HEE1
CSB-EP321038HEE1
CSB-BP321038HEE1
CSB-MP321038HEE1
CSB-EP321038HEE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Woodchuck hepatitis B virus Protein X (X) CSB-YP321039WAT
CSB-EP321039WAT
CSB-BP321039WAT
CSB-MP321039WAT
CSB-EP321039WAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Periplasmic [NiFe] hydrogenase small subunit (hydA) CSB-YP321040DJO
CSB-EP321040DJO
CSB-BP321040DJO
CSB-MP321040DJO
CSB-EP321040DJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATP-dependent DNA helicase SRS2 (SRS2), partial CSB-YP321041SVG
CSB-EP321041SVG
CSB-BP321041SVG
CSB-MP321041SVG
CSB-EP321041SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human X-ray repair cross-complementing protein 6 (XRCC6) CSB-YP321042HU
CSB-EP321042HU
CSB-BP321042HU
CSB-MP321042HU
CSB-EP321042HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methyl-coenzyme M reductase subunit alpha (mcrA), partial CSB-YP321043MFF
CSB-EP321043MFF
CSB-BP321043MFF
CSB-MP321043MFF
CSB-EP321043MFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heliothis virescens Juvenile hormone esterase, partial CSB-YP321044HVF
CSB-EP321044HVF
CSB-BP321044HVF
CSB-MP321044HVF
CSB-EP321044HVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Biotin synthase (bioB) CSB-YP321045ENV
CSB-EP321045ENV
CSB-BP321045ENV
CSB-MP321045ENV
CSB-EP321045ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus phage phivML3 Uncharacterized protein near lysin gene CSB-YP321046LBL
CSB-EP321046LBL
CSB-BP321046LBL
CSB-MP321046LBL
CSB-EP321046LBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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