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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Influenza A virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP321448IHK
CSB-EP321448IHK
CSB-BP321448IHK
CSB-MP321448IHK
CSB-EP321448IHK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage phi29 Early protein GP16.8 (16.8) CSB-YP321449BBC
CSB-EP321449BBC
CSB-BP321449BBC
CSB-MP321449BBC
CSB-EP321449BBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella haemolytica Leukotoxin secretion protein D (lktD), partial CSB-YP321450ESE
CSB-EP321450ESE
CSB-BP321450ESE
CSB-MP321450ESE
CSB-EP321450ESE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Granaticin polyketide synthase putative ketoacyl reductase 1 (gra-orf5) CSB-YP321451SQH
CSB-EP321451SQH
CSB-BP321451SQH
CSB-MP321451SQH
CSB-EP321451SQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha-galactosidase (rafA) CSB-YP321452ENL
CSB-EP321452ENL
CSB-BP321452ENL
CSB-MP321452ENL
CSB-EP321452ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetracenomycin polyketide synthesis protein tcmJ (tcmJ) CSB-YP321453SOG
CSB-EP321453SOG
CSB-BP321453SOG
CSB-MP321453SOG
CSB-EP321453SOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein bicaudal D (BicD), partial CSB-YP321454DLU
CSB-EP321454DLU
CSB-BP321454DLU
CSB-MP321454DLU
CSB-EP321454DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon Allophycocyanin alpha chain 2 (apcA2) CSB-YP321455FCY
CSB-EP321455FCY
CSB-BP321455FCY
CSB-MP321455FCY
CSB-EP321455FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mumps virus Phosphoprotein (P/V) CSB-YP321456MJJ
CSB-EP321456MJJ
CSB-BP321456MJJ
CSB-MP321456MJJ
CSB-EP321456MJJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative uncharacterized 9.3 kDa protein CSB-YP321457MBE
CSB-EP321457MBE
CSB-BP321457MBE
CSB-MP321457MBE
CSB-EP321457MBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum Endoglucanase S (celS) CSB-YP321458ESP
CSB-EP321458ESP
CSB-BP321458ESP
CSB-MP321458ESP
CSB-EP321458ESP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Glycoprotein 24A (csbA) CSB-YP321459DKK
CSB-EP321459DKK
CSB-BP321459DKK
CSB-MP321459DKK
CSB-EP321459DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus S Movement and silencing protein TGBp1 (ORF2) CSB-YP321460PRB
CSB-EP321460PRB
CSB-BP321460PRB
CSB-MP321460PRB
CSB-EP321460PRB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Uncharacterized protein ycjE (ycjE) CSB-YP321461SXB
CSB-EP321461SXB
CSB-BP321461SXB
CSB-MP321461SXB
CSB-EP321461SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Antigen TpF1 (tpf1) CSB-YP321462TPR
CSB-EP321462TPR
CSB-BP321462TPR
CSB-MP321462TPR
CSB-EP321462TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein phnB (phnB) CSB-YP321463ENV
CSB-EP321463ENV
CSB-BP321463ENV
CSB-MP321463ENV
CSB-EP321463ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein phnM (phnM) CSB-YP321464ENV
CSB-EP321464ENV
CSB-BP321464ENV
CSB-MP321464ENV
CSB-EP321464ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Uteroferrin-associated protein CSB-YP321465PI
CSB-EP321465PI
CSB-BP321465PI
CSB-MP321465PI
CSB-EP321465PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheeppox virus Viral replication protein A28-like (HM3), partial CSB-YP321466SFW
CSB-EP321466SFW
CSB-BP321466SFW
CSB-MP321466SFW
CSB-EP321466SFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Virion-packaging protein UL77 (UL77), partial CSB-YP321467HWV
CSB-EP321467HWV
CSB-BP321467HWV
CSB-MP321467HWV
CSB-EP321467HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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