Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Inhibitory regulator protein IRA1 (IRA1), partial CSB-YP321652SVG
CSB-EP321652SVG
CSB-BP321652SVG
CSB-MP321652SVG
CSB-EP321652SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Iguana iguana Hemoglobin subunit alpha-1 CSB-YP321653IAG
CSB-EP321653IAG
CSB-BP321653IAG
CSB-MP321653IAG
CSB-EP321653IAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uromastyx hardwickii Hemoglobin subunit alpha-1 CSB-YP321654UAV
CSB-EP321654UAV
CSB-BP321654UAV
CSB-MP321654UAV
CSB-EP321654UAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Vitellogenin-B1 CSB-YP321656XBE
CSB-EP321656XBE
CSB-BP321656XBE
CSB-MP321656XBE
CSB-EP321656XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Cell division control protein 2 homolog 1 (CDC2) CSB-YP321657EWE
CSB-EP321657EWE
CSB-BP321657EWE
CSB-MP321657EWE
CSB-EP321657EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline immunodeficiency virus Uncharacterized protein ORF3 CSB-YP321658FAM
CSB-EP321658FAM
CSB-BP321658FAM
CSB-MP321658FAM
CSB-EP321658FAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus vulcanus Photosystem II reaction center protein H (psbH), partial CSB-YP321659TNI
CSB-EP321659TNI
CSB-BP321659TNI
CSB-MP321659TNI
CSB-EP321659TNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Reaction center protein L chain (pufL), partial CSB-YP321660RLC1
CSB-EP321660RLC1
CSB-BP321660RLC1
CSB-MP321660RLC1
CSB-EP321660RLC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas sp. 2-hydroxymuconic semialdehyde hydrolase (dmpD) CSB-YP321661PVK
CSB-EP321661PVK
CSB-BP321661PVK
CSB-MP321661PVK
CSB-EP321661PVK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Chorismate mutase AroH (aroH) CSB-YP321662BRJ
CSB-EP321662BRJ
CSB-BP321662BRJ
CSB-MP321662BRJ
CSB-EP321662BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coat protein CSB-YP321663CAC
CSB-EP321663CAC
CSB-BP321663CAC
CSB-MP321663CAC
CSB-EP321663CAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumis sativus Peroxidase 2 CSB-YP321664CXP
CSB-EP321664CXP
CSB-BP321664CXP
CSB-MP321664CXP
CSB-EP321664CXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris Phenylalanine ammonia-lyase class 3, partial CSB-YP321665EUN
CSB-EP321665EUN
CSB-BP321665EUN
CSB-MP321665EUN
CSB-EP321665EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces sp. Xylose isomerase (xylA) CSB-YP321666SPV
CSB-EP321666SPV
CSB-BP321666SPV
CSB-MP321666SPV
CSB-EP321666SPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein J (psbJ), partial CSB-YP321667DZX
CSB-EP321667DZX
CSB-BP321667DZX
CSB-MP321667DZX
CSB-EP321667DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flaveria bidentis Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP321668FBM
CSB-EP321668FBM
CSB-BP321668FBM
CSB-MP321668FBM
CSB-EP321668FBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S19, chloroplastic (rps19) CSB-YP321669EHU
CSB-EP321669EHU
CSB-BP321669EHU
CSB-MP321669EHU
CSB-EP321669EHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia phage 1 Protein ORF5 (ORF5) CSB-YP321670CIV
CSB-EP321670CIV
CSB-BP321670CIV
CSB-MP321670CIV
CSB-EP321670CIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia phage 1 Minor spike protein H (ORF2) CSB-YP321671CIV
CSB-EP321671CIV
CSB-BP321671CIV
CSB-MP321671CIV
CSB-EP321671CIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica Invasin, partial CSB-YP321672YAQ
CSB-BP321672YAQ
CSB-MP321672YAQ
CSB-EP321672YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>